Rabbit DAP3 Polyclonal Antibody | anti-DAP3 antibody

DAP3 Antibody

Cat. Num. AAA9612149

• Gene Names: DAP3; DAP-3; S29mt; MRPS29; MRP-S29; bMRP-10
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: DAP3; Polyclonal Antibody; DAP3 Antibody; 28S ribosomal protein S29; 28S ribosomal protein S29 mitochondrial; bMRP10; DAP 3; DAP-3; Dap3; Death associated protein 3; Death-associated protein 3; DKFZp686G12159; FLJ12817; Ionizing radiation resistance conferring protein; MGC126058; MGC126059; Mitochondrial 28S ribosomal protein S29; mitochondrial; MITOCHONDRIAL RIBOSOMAL PROTEIN S29; MRP S29; MRP-S29; MRPS29; OTTHUMP00000033480; OTTHUMP00000033481; PSD-95/SAP90-binding protein 3; RT29_HUMAN; S29mt; SAP90/PSD-95-associated protein 3; SAPAP3; anti-DAP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DAP3 Antibody detects endogenous levels of total DAP3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 398

• Applicable Applications for anti-DAP3 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human DAP3, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from MOLT4, using DAP3 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-DAP3 antibody

Function: Involved in mediating interferon-gamma-induced cell death.

Subcellular Location: Mitochondrion.

Tissue Specificity: Ubiquitous.

Subunit Structure: Component of the mitochondrial small ribosomal subunit (mt-SSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins (PubMed:25838379). Interacts with DELE1 (PubMed:20563667). Interacts with NOA1 (PubMed:19103604).

Similarity: Belongs to the mitochondrion-specific ribosomal protein mS29 family.

NCBI and Uniprot Product Information

• NCBI GI #: 1706299
• NCBI GeneID: 7818
• UniProt Accession #: P51398
• Molecular Weight: Observed Molecular Weight: (Observed)45kD.; Predicted Molecular Weight: (Calculated)46kDa.
• NCBI Official Full Name: 28S ribosomal protein S29, mitochondrial
• NCBI Official Synonym Full Names: death associated protein 3
• NCBI Official Symbol: DAP3
• NCBI Official Synonym Symbols: DAP-3; S29mt; MRPS29; MRP-S29; bMRP-10
• NCBI Protein Information: 28S ribosomal protein S29, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S29, mitochondrial
• Protein Family: 28S ribosomal protein
• UniProt Gene Name: DAP3
• UniProt Synonym Gene Names: MRPS29; MRP-S29; S29mt; DAP-3
• UniProt Entry Name: RT29_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Dec 2010]

Uniprot Description

DAP3: Involved in mediating interferon-gamma-induced cell death. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: nucleoplasm; small ribosomal subunit; mitochondrion; mitochondrial inner membrane; mitochondrial ribosome; nucleolus; nucleus

Molecular Function: protein binding

Biological Process: apoptotic mitochondrial changes; mitochondrial translation; organelle organization and biogenesis