Rabbit Dab1 Polyclonal Antibody | anti-Dab1 antibody

Dab1 (Phospho-Y232) Polyclonal Antibody

Cat. Num. AAA3005114

• Gene Names: DAB1; SCA37
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Dab1; Polyclonal Antibody; Dab1 (Phospho-Y232) Polyclonal Antibody; Disabled homolog 1; DAB1; anti-Dab1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-Dab1 (Y232) polyclonal antibody detects endogenous levels of Dab1 protein when phosphorylated at Tyr232
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 555

• Applicable Applications for anti-Dab1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human Dab1 around the phosphorylation site of Tyrosine 232.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Dab1 (phospho-Y232) polyclonal antibody at 1:1000 dilution Lane1:LO2 whole cell lysate (38ug) Lane2:The Brain tissue lysate of Rat (44ug) Lane3:The Brain tissue lysate of Mouse (39ug))

Related Product Information for anti-Dab1 antibody

Dab1, a homolog of the Drosophila Disabled protein, is an adaptor protein involved in neural development. This cytoplasmic protein is tyrosine-phosphorylated during rapid expansion of the developing nervous system, and it is thought to interact with other proteins via a domain similar to the PTB domains of the Shc family. Dab1 has been shown to interact with the SH2 domains of Src, Fyn and Abl. Mutations in Dab1 result in widespread abnormalities in the brain, similar to those seen in Reelin mutants. Reelin is a secreted protein thought to play a role in directing migrating neurons. Evidence suggests that Dab1 functions downstream of Reelin in a signaling pathway involved in positioning cells in the developing brain

NCBI and Uniprot Product Information

• NCBI GI #: 33350928
• NCBI GeneID: 1600
• NCBI Accession #: NP_066566.3
• NCBI GenBank Nucleotide #: NP_066566.3
• UniProt Accession #: O75553
• Molecular Weight: ~ 60kDa
• NCBI Official Full Name: disabled homolog 1 isoform 1
• NCBI Official Synonym Full Names: DAB adaptor protein 1
• NCBI Official Symbol: DAB1
• NCBI Official Synonym Symbols: SCA37
• NCBI Protein Information: disabled homolog 1
• UniProt Protein Name: Disabled homolog 1
• Protein Family: Disabled
• UniProt Gene Name: DAB1
• UniProt Entry Name: DAB1_HUMAN

NCBI Description

The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. [provided by RefSeq, Jan 2017]

Uniprot Description

DAB1: an adaptor molecule functioning in neural development. The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. Thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Associates with the SH2 domains of Src, Fyn and Abl. Phosphorylated in response to reelin induction in embryonic neurons

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: neuron projection; cell soma; membrane; apical part of cell; perinuclear region of cytoplasm; postsynaptic density; cytosol; brush border

Molecular Function: phosphoinositide 3-kinase binding

Biological Process: cell-cell adhesion involved in neuronal-glial interactions involved in cerebral cortex radial glia guided migration; response to drug; Golgi localization; cerebellum structural organization; ventral spinal cord development; negative regulation of cell adhesion; adult walking behavior; small GTPase mediated signal transduction; radial glia guided migration of Purkinje cell; positive regulation of protein kinase activity; negative regulation of astrocyte differentiation; dendrite development; midgut development; negative regulation of axonogenesis; cerebral cortex radially oriented cell migration; positive regulation of neuron differentiation; negative regulation of JAK-STAT cascade