Rabbit DAAM1 Polyclonal Antibody | anti-DAAM1 antibody

DAAM1 (Disheveled-associated Activator of Morphogenesis 1, KIAA0666)

Cat. Num. AAA6011917

• Reactivity: Bovine, Canine, Equine, Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: DAAM1; Polyclonal Antibody; DAAM1 (Disheveled-associated Activator of Morphogenesis 1; KIAA0666); Anti -DAAM1 (Disheveled-associated Activator of Morphogenesis 1; anti-DAAM1 antibody

• Host: Rabbit
• Reactivity: Bovine, Canine, Equine, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DAAM1. Species Crossreactivity: bovine, canine, equine, mouse, rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

• Applicable Applications for anti-DAAM1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 4-6ug/ml
• Immunogen: Synthetic peptide corresponding to aa1-50 of human DAAM1.
• Positive Control: Human skeletal muscle lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DAAM1 in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide using MBS6011917 at 5ug/ml.)

Related Product Information for anti-DAAM1 antibody

DAAM1 is a diaphanous-related formin first studied as a novel Dishevelled binding protein and shown to be crucial for the planar cell polarity pathway in Xenopus. DAM1, like other formins, directs nucleation and elongation of new actin filaments using its conserved formin-homology-2 (FH2) domain. It is a Rho-regulated formin implicated in neuronal development and regulation of gastrulation. It was identified as a binding protein of the Wnt receptor-associated protein Dishevelled. It also binds the GTP-bound RhoA and increases its GTP-bound form population in cells. It exists in an autoinhibited state via intramolecular interactions between its amino-terminal GBD and carboxyl-terminalDD domains. Structurally the protein includes a GTPase-binding domain (GBD), a diaphanous inhibitory domain (DID), and a coiled-coil region followed by the FH1, FH2 and C-terminal diaphanous auto-regulatory domain (DAD). In humans, theDAM1 gene is mapped to chromosome 14q23.1.

Product Categories/Family for anti-DAAM1 antibody

Antibodies; Abs to Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 395394053
• NCBI GeneID: 23002
• NCBI Accession #: NP_001257449.1
• NCBI GenBank Nucleotide #: NM_001270520.1
• UniProt Accession #: Q9Y4D1; Q86U34; Q8N1Z8; Q8TB39
• Molecular Weight: 123,473 Da
• NCBI Official Full Name: disheveled-associated activator of morphogenesis 1 isoform 2
• NCBI Official Synonym Full Names: dishevelled associated activator of morphogenesis 1
• NCBI Official Symbol: DAAM1
• NCBI Protein Information: disheveled-associated activator of morphogenesis 1
• UniProt Protein Name: Disheveled-associated activator of morphogenesis 1
• Protein Family: Disheveled-associated activator of morphogenesis
• UniProt Gene Name: DAAM1
• UniProt Synonym Gene Names: KIAA0666
• UniProt Entry Name: DAAM1_HUMAN

NCBI Description

Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2012]

Uniprot Description

DAAM1: Binds to disheveled (Dvl) and Rho, and mediates Wnt- induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Belongs to the formin homology family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: membrane; cytoplasm; stress fiber; plasma membrane; cytosol

Molecular Function: identical protein binding; protein binding; Rho GTPase binding; actin binding

Biological Process: actin cytoskeleton organization and biogenesis