Goat Cytohesin 2 Polyclonal Antibody | anti-CYTH2 antibody

Cytohesin 2 (ARF Exchange Factor, ARF Nucleotide-binding Site Opener, ARNO Protein, CTS18.1, Pleckstrin Homology Sec7 and Coiled-Coil Domains 2, PSCD2, Sec7p-L)

Cat. Num. AAA622111

• Gene Names: CYTH2; ARNO; CTS18; PSCD2; SEC7L; PSCD2L; CTS18.1; Sec7p-L; Sec7p-like
• Reactivity: Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Cytohesin 2; Polyclonal Antibody; Cytohesin 2 (ARF Exchange Factor; ARF Nucleotide-binding Site Opener; ARNO Protein; CTS18.1; Pleckstrin Homology Sec7 and Coiled-Coil Domains 2; PSCD2; Sec7p-L); Anti -Cytohesin 2 (ARF Exchange Factor; anti-CYTH2 antibody

• Host: Goat
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes ARNO at ~48kD in Human Brain (Cerebellum) and Rat Brain lysates (calculated MW of 46.5kD according to NP_004219.2 and NP_059431.1). This antibody is expected to recognize both reported isoforms (NP_004219.2; NP_059431.1). Species Crossreactivity: Canine, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-CYTH2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:32000; Western Blot: 0.5-1.5ug/ml
• Immunogen: Synthetic peptide of ARNO.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of ARNO in Human Brain (Cerebellum) lysate (35μg protein in RIPA buffer) using ARNO antibody (0.5μg/ml).)

Related Product Information for anti-CYTH2 antibody

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21kD proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. Like other small GTPases, Arf is found to be active when bound to GTP and inactive when bound to GDP. Arf?s activation is dependent upon guanine nucleotide exchange factors (GEFs) which increase the rate of exchange of bound GDP with GTP. All GEFs have a highly conserved Sec7 domain. GEF activity lies in the Sec7 domain and this activity has been shown to be inhibited by the fungal metabolite brefeldin-A (BFA). A small group of GEFs which are insensitive to brefeldin-A (BFA) include cytohesin-1 (B2-1), cytohesin-2 (ARNO), cytohesin-3 (ARNO3), and cytohesin-4. All cytohesins function in the cell periphery and contain a pleckstrin homology (PH) domain. The PH domain has been shown to interact with phosphatidylinositol 3,4,5-triphosphate and is believed to promote membrane targeting of the cytohesins. Recruitment of the cytohesins to the membranes can occur in response to tyrosine kinase receptor activation. This response appears to require the activation of phosphatidylinositol 3-kinase (PI 3-kinase).

Product Categories/Family for anti-CYTH2 antibody

Antibodies; Abs to Pleckstrin Homology Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 195972859
• NCBI GeneID: 9266
• NCBI Accession #: NP_004219.3
• NCBI GenBank Nucleotide #: NM_004228.6
• UniProt Accession #: Q59F87; Q8WYS2; Q99418
• Molecular Weight: 27,990 Da
• NCBI Official Full Name: cytohesin-2 isoform 2
• NCBI Official Synonym Full Names: cytohesin 2
• NCBI Official Symbol: CYTH2
• NCBI Official Synonym Symbols: ARNO; CTS18; PSCD2; SEC7L; PSCD2L; CTS18.1; Sec7p-L; Sec7p-like
• NCBI Protein Information: cytohesin-2; OTTHUMP00000196753; OTTHUMP00000196754; ARF exchange factor; ARF nucleotide-binding site opener; PH, SEC7 and coiled-coil domain-containing protein 2; pleckstrin homology, Sec7 and coiled-coil domains 2 (cytohesin-2); pleckstrin homology, Sec7 and coiled/coil domains 2 (cytohesin-2)
• UniProt Protein Name: Pleckstrin homology, Sec7 and coiled/coil domains 2 isoform 2 variant
• Protein Family: Cytohesin
• UniProt Entry Name: Q59F87_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with ARF1, ARF3, and ARF6 and is 83% homologous to CYTH1. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

cytohesin 2: Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, ARF; GEFs

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: membrane; cytoplasm; plasma membrane; trans-Golgi network

Molecular Function: protein binding; lipid binding; ARF guanyl-nucleotide exchange factor activity

Biological Process: vesicle-mediated transport; regulation of cell adhesion; endocytosis; regulation of ARF protein signal transduction; actin cytoskeleton organization and biogenesis; positive regulation of GTPase activity