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Western Blot (WB) (Western blot analysis of extracts from HepG2, using Cytochrome P450 2A13 Antibody. Lane 1 was treated with the antigen-specific peptide.)

Rabbit anti-Human, Rat Cytochrome P450 2A13 Polyclonal Antibody | anti-CYP2A13 antibody

Cytochrome P450 2A13 Antibody

Gene Names
CYP2A13; CPAD; CYP2A; CYPIIA13
Reactivity
Human, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Cytochrome P450 2A13; Polyclonal Antibody; Cytochrome P450 2A13 Antibody; CP2AD_HUMAN; CPAD; CYP2A; CYP2A13; CYPIIA13; CYTOCHROME P450; SUBFAMILY IIA; POLYPEPTIDE 13; anti-CYP2A13 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Cytochrome P450 2A13 antibody detects endogenous levels of total Cytochrome P450 2A13
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
494
Applicable Applications for anti-CYP2A13 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Endoplasmic Reticulum Membrane. Microsome Membrane.
Tissue Specificity
Expressed in liver and a number of extrahepatic tissues, including nasal mucosa, lung, trachea, brain, mammary gland, prostate, testis, and uterus, but not in heart, kidney, bone marrow, colon, small intestine, spleen, stomach, thymus, or skeletal muscle.
Predicted Cross Reactivity
Horse, Dog
Similarity
Horse (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2, using Cytochrome P450 2A13 Antibody. Lane 1 was treated with the antigen-specific peptide.)

Western Blot (WB) (Western blot analysis of extracts from HepG2, using Cytochrome P450 2A13 Antibody. Lane 1 was treated with the antigen-specific peptide.)

Immunofluorescene (IF)

(MBS9605029 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Immunofluorescene (IF) (MBS9605029 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells using Cytochrome P450 2A13 antibody.The lane on the left is treated with the antigen-specific peptide.)

Western Blot (WB) (Western blot analysis of extracts from HeLa cells using Cytochrome P450 2A13 antibody.The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-CYP2A13 antibody
Function: Exhibits a coumarin 7-hydroxylase activity. Active in the metabolic activation of hexamethylphosphoramide, N, N-dimethylaniline, 2'-methoxyacetophenone, N-nitrosomethylphenylamine, and the tobacco-specific carcinogen, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. Possesses phenacetin O-deethylation activity.
Similarity: Belongs to the cytochrome P450 family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 56 kD
Predicted: 57 kDa
NCBI Official Full Name
cytochrome P450 2A13
NCBI Official Synonym Full Names
cytochrome P450 family 2 subfamily A member 13
NCBI Official Symbol
CYP2A13
NCBI Official Synonym Symbols
CPAD; CYP2A; CYPIIA13
NCBI Protein Information
cytochrome P450 2A13
UniProt Protein Name
Cytochrome P450 2A13
Protein Family
UniProt Gene Name
CYP2A13

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. Although its endogenous substrate has not been determined, it is known to metabolize 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, a major nitrosamine specific to tobacco. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]

Uniprot Description

Exhibits a coumarin 7-hydroxylase activity. Active in the metabolic activation of hexamethylphosphoramide, N,N-dimethylaniline, 2'-methoxyacetophenone, N-nitrosomethylphenylamine, and the tobacco-specific carcinogen, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. Possesses phenacetin O-deethylation activity.

Research Articles on CYP2A13

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Product Notes

The CYP2A13 cyp2a13 (Catalog #AAA9605029) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cytochrome P450 2A13 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 2A13 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the CYP2A13 cyp2a13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 2A13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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