Western Blot (WB)
(Western blot analysis of extracts from COLO205 cells, usingCytochrome P450 11B1/2 Antibody. The lane on the left wastreated with blocking peptide.)
Rabbit anti-Human Cytochrome P450 11B1/2 Polyclonal Antibody
Rabbit Cytochrome P450 11B1/2 Antibody
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by affinity chromatography using SulfoLink Coupling Resin
Synonyms
Cytochrome P450 11B1/2; Polyclonal Antibody; Rabbit Cytochrome P450 11B1/2 Antibody; anti-Cytochrome P450 11B1/2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Cytochrome P450 11B1/2 Antibody detects endogenous levels of total Cytochrome P450 11B1/2
Purity/Purification
The antiserum was purified by affinity chromatography using SulfoLink Coupling Resin
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-Cytochrome P450 11B1/2 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
ELISA(peptide): 1:20000-1:40000
Important: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking,overnight.
IF/ICC: 1:100-1:500
ELISA(peptide): 1:20000-1:40000
Important: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking,overnight.
Immunogen
A synthesized peptide derived from human Cytochrome P450 11B1/2, corresponding to a region within N-terminal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from COLO205 cells, usingCytochrome P450 11B1/2 Antibody. The lane on the left wastreated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from COLO205 cells, usingCytochrome P450 11B1/2 Antibody. The lane on the left wastreated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from COLO205 cells, usingCytochrome P450 11B1/2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from COLO205 cells, usingCytochrome P450 11B1/2 antibody.)
Immunofluorescence (IF)
(Staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(Staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
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Product Notes
The Cytochrome P450 11B1/2 (Catalog #AAA003338) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Cytochrome P450 11B1/2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 11B1/2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB: 1:500-1:1000 IF/ICC: 1:100-1:500 ELISA(peptide): 1:20000-1:40000 Important: For western blot, incubate membrane with diluted primary Abin 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking,overnight. Researchers should empirically determine the suitability of the Cytochrome P450 11B1/2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 11B1/2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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