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Western Blot (WB)
(Western blot analysis of extracts from rat kidney, using Cytochrome c-type Heme Lyase Antibody.)
Rabbit Cytochrome c-type Heme Lyase Polyclonal Antibody | anti-HCCS antibody
Cytochrome c-type Heme Lyase Antibody
Gene Names
HCCS; MLS; CCHL; MCOPS7; LSDMCA1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Peptide affinity purification
Synonyms
Cytochrome c-type Heme Lyase; Polyclonal Antibody; Cytochrome c-type Heme Lyase Antibody; CCHL; CCHL_HUMAN; cytochrome c heme-lyase; Cytochrome c-type heme lyase; DKFZp779I1858; EC 4.4.1.17; Hccs; Holocytochrome c synthase (cytochrome c heme lyase); Holocytochrome c synthase; Holocytochrome c type synthase; Holocytochrome c-type synthase; MCOPS7; OTTHUMP00000022903; OTTHUMP00000022904; OTTHUMP00000022905; OTTMUSP00000021173; OTTMUSP00000021174; RGD1563855; RP23-37L2.1; anti-HCCS antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Cytochrome c-type Heme Lyase Antibody detects endogenous levels of total Cytochrome c-type Heme Lyase.
Purity/Purification
Peptide affinity purification
Form/Format
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-HCCS antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB 1:500-1:1000
IHC: 1:50-1:200
IF/ICC 1:100-1:500
ELISA(peptide): 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IHC: 1:50-1:200
IF/ICC 1:100-1:500
ELISA(peptide): 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human Cytochrome ctype Heme Lyase, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C.
Western Blot (WB)
(Western blot analysis of extracts from rat kidney, using Cytochrome c-type Heme Lyase Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from rat kidney, using Cytochrome c-type Heme Lyase Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells using Cytochrome c-type Heme Lyase antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells using Cytochrome c-type Heme Lyase antibody.)
Immunofluorescence (IF)
(MBS9604997 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9604997 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9604997 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9604997 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-HCCS antibody
Function: Links covalently the heme group to the apoprotein of cytochrome c.
Similarity: Belongs to the cytochrome c-type heme lyase family.
Similarity: Belongs to the cytochrome c-type heme lyase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31 KD
NCBI Official Full Name
cytochrome c-type heme lyase
NCBI Official Synonym Full Names
holocytochrome c synthase
NCBI Official Symbol
HCCS
NCBI Official Synonym Symbols
MLS; CCHL; MCOPS7; LSDMCA1
NCBI Protein Information
cytochrome c-type heme lyase
UniProt Protein Name
Cytochrome c-type heme lyase
Protein Family
UniProt Gene Name
HCCS
UniProt Synonym Gene Names
CCHL; CCHL
NCBI Description
The protein encoded by this gene is an enzyme that covalently links a heme group to the apoprotein of cytochrome c. Defects in this gene are a cause of microphthalmia syndromic type 7 (MCOPS7). Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2010]
Uniprot Description
Links covalently the heme group to the apoprotein of cytochrome c.
Research Articles on HCCS
Similar Products
Product Notes
The HCCS hccs (Catalog #AAA9604997) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cytochrome c-type Heme Lyase Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome c-type Heme Lyase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB 1:500-1:1000 IHC: 1:50-1:200 IF/ICC 1:100-1:500 ELISA(peptide): 1:20000-1:40000 *The optimal dilutions should be determined by the end user. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the HCCS hccs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome c-type Heme Lyase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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