Rabbit Vascular Endothelial Growth Factor A (VEGF-A) ELISA Kit | VEGF-A elisa kit

Rabbit Vascular Endothelial Growth Factor A (VEGF-A) ELISA Kit

Cat. Num. AAA2601341

• Gene Names: VEGFA; VPF; VEGF; MVCD1
• Reactivity: Rabbit
• Synonyms: Vascular Endothelial Growth Factor A (VEGF-A); Rabbit Vascular Endothelial Growth Factor A (VEGF-A) ELISA Kit; VEGF-A elisa kit

• Reactivity: Rabbit
• Specificity: No cross-reaction with other factors.
• Sequence Length: 232

• Samples: Rabbit Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Va Concentration
• Assay Type: Sandwich
• Detection Range: 10 ng/ml-0.156ng/ml
• Sensitivity: 0.05 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGF-A elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rabbit VA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 17380528
• NCBI GeneID: 7422
• Molecular Weight: 34,406 Da
• NCBI Official Full Name: Vascular endothelial growth factor A
• NCBI Official Synonym Full Names: vascular endothelial growth factor A
• NCBI Official Symbol: VEGFA
• NCBI Official Synonym Symbols: VPF; VEGF; MVCD1
• NCBI Protein Information: vascular endothelial growth factor A; vascular permeability factor
• UniProt Protein Name: Vascular endothelial growth factor A
• UniProt Gene Name: VEGFA
• UniProt Synonym Gene Names: VEGF; VEGF-A; VPF
• UniProt Entry Name: VEGFA_HUMAN

NCBI Description

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.