Rabbit Cytochrome C Polyclonal Antibody | anti-CYCS antibody

Anti-Cytochrome C antibody

Cat. Num. AAA175535

• Gene Names: CYCS; CYC; HCS; THC4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Cytochrome C; Polyclonal Antibody; Anti-Cytochrome C antibody; Cytochrome c; cytochrome c; somatic; CYC antibody; CYC_HUMAN antibody; CYCS antibody; Cytochrome c antibody; Cytochrome c somatic antibody; HCS antibody; THC4 antibody; anti-CYCS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 105

• Applicable Applications for anti-CYCS antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cytochrome C (91-105 aa ERADLIAYLKKATNE), identical to the related mouse and rat sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-Cytochrome C antibody, MBS175535, IHC(P)IHC(P): Rat Lung Tissue )

Immunohistochemistry (IHC)

(Anti-Cytochrome C antibody, MBS175535, IHC(F)IHC(F): Rat Brain Tissue )

Immunocytochemistry (ICC)

(Anti-Cytochrome C antibody, MBS175535, ICCICC: A549 Cell )

Western Blot (WB)

(Anti-Cytochrome C antibody, MBS175535, Western blottingAll lanes: Anti Cytochrome C (MBS175535) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: MCF-7 Whole Cell Lysate at 40ugLane 4: HEPA Whole Cell Lysate at 40ugPredicted bind size: 12KDObserved bind size: 12KD )

Related Product Information for anti-CYCS antibody

Description: Rabbit IgG polyclonal antibody for Cytochrome c(CYCS) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.
Background: Cytochrome C is located in the mitochondria of all aerobic cells and is involved in the electron transport system. Human cytochrome c has 104 amino acid residues and a molecular weight of 11,458 and is mapped to 7p15.2. Cytochrome c released from mitochondria has been proposed to be an essential component of an apoptotic pathway responsive to DNA damage and other forms of cell stress. And it has a role in different apoptotic signaling cascades.

NCBI and Uniprot Product Information

• NCBI GI #: 42560196
• NCBI GeneID: 54205
• UniProt Accession #: P99999; P00001; Q6NUR2; Q6NX69; Q96BV4; A4D166; B2R4I1
• Molecular Weight: 11,749 Da
• NCBI Official Full Name: Cytochrome c
• NCBI Official Synonym Full Names: cytochrome c, somatic
• NCBI Official Symbol: CYCS
• NCBI Official Synonym Symbols: CYC; HCS; THC4
• NCBI Protein Information: cytochrome c
• UniProt Protein Name: Cytochrome c
• Protein Family: Cytochrome
• UniProt Gene Name: CYCS
• UniProt Synonym Gene Names: CYC
• UniProt Entry Name: CYC_HUMAN

NCBI Description

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]

Uniprot Description

CYCS: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Belongs to the cytochrome c family.

Protein type: Oxidoreductase; Protein phosphatase, Ser/Thr (non-receptor); Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 7p15.3

Cellular Component: protein phosphatase type 2A complex; mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; nucleus; cytosol

Molecular Function: electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; protein binding; iron ion binding; heme binding; protein serine/threonine phosphatase activity

Biological Process: cellular metabolic process; mitochondrion organization and biogenesis; response to reactive oxygen species; dephosphorylation; apoptosis; organelle organization and biogenesis; cellular respiration; mitochondrial electron transport, cytochrome c to oxygen; caspase activation via cytochrome c; DNA fragmentation during apoptosis; transmembrane transport; mitochondrial electron transport, ubiquinol to cytochrome c

Disease: Thrombocytopenia 4