Western Blot (WB)
((0.1ug/ml)MBS627271 staining of Mouse Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat anti-Mouse Cytochrome b5 Reductase 3, CYB5R3 Polyclonal Antibody | anti-CYB5R3 antibody
Cytochrome b5 Reductase 3, CYB5R3 (Diaphorase 1 (NADH), diaphorase 1, NADH-cytochrome b5 reductase, WU:Cyb5r3, WU:AL591952.1-003, WU:AL591952.1-002, WU:AL591952.1-001, Dia1, Dia-1, C85115, 2500002N19Rik, 0610016L08Rik, cytochrome b5 reductase 3, Cyb5r3)
Purified by peptide affinity chromatography.
Purified by peptide affinity chromatography.
Dilution: Peptide ELISA Titer: 1:32,000
Western Blot: 0.1-1ug/ml detects a ~35kD band observed in Mouse Liver lysates as well as in mouse and rat brain lysates (calculated MW of 34.1kD according to mouse NP_084063.1).
Western Blot (WB)
((0.1ug/ml)MBS627271 staining of Mouse Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
((0.1ug/ml)MBS627271 staining of Mouse Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq]
Uniprot Description
CYB5R3: Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Defects in CYB5R3 are the cause of methemoglobinemia CYB5R3-related (METHB-CYB5R3). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. There are two types of methemoglobinemia CYB5R3-related. In type 1, the defect affects the soluble form of the enzyme, is restricted to red blood cells, and causes well- tolerated methemoglobinemia. In type 2, the defect affects both the soluble and microsomal forms of the enzyme and is thus generalized, affecting red cells, leukocytes and all body tissues. Type 2 methemoglobinemia is associated with mental deficiency and other neurologic symptoms. Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. 3 isoforms of the human protein are produced by alternative promoter.
Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Oxidoreductase; EC 1.6.2.2; Mitochondrial
Chromosomal Location of Human Ortholog: 22q13.2
Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; mitochondrion; membrane; hemoglobin complex; endoplasmic reticulum; mitochondrial inner membrane; cytoplasm; lipid particle
Molecular Function: FAD binding; cytochrome-b5 reductase activity; ADP binding; NAD binding; AMP binding
Biological Process: vitamin metabolic process; blood circulation; L-ascorbic acid metabolic process; cholesterol biosynthetic process; water-soluble vitamin metabolic process
Disease: Methemoglobinemia Due To Deficiency Of Methemoglobin Reductase
Research Articles on CYB5R3
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Product Notes
The CYB5R3 cyb5r3 (Catalog #AAA627271) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Cytochrome b5 Reductase 3, CYB5R3 (Diaphorase 1 (NADH), diaphorase 1, NADH-cytochrome b5 reductase, WU:Cyb5r3, WU:AL591952.1-003, WU:AL591952.1-002, WU:AL591952.1-001, Dia1, Dia-1, C85115, 2500002N19Rik, 0610016L08Rik, cytochrome b5 reductase 3, Cyb5r3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome b5 Reductase 3, CYB5R3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Peptide ELISA Titer: 1:32,000 Western Blot: 0.1-1ug/ml detects a ~35kD band observed in Mouse Liver lysates as well as in mouse and rat brain lysates (calculated MW of 34.1kD according to mouse NP_084063.1). Researchers should empirically determine the suitability of the CYB5R3 cyb5r3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome b5 Reductase 3, CYB5R3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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