Rabbit anti-Human CYTH4 Polyclonal Antibody | anti-CYTH4 antibody

CYTH4, CT (CYTH4, CYT4, PSCD4, Cytohesin-4, PH, SEC7 and coiled-coil domain-containing protein 4) (APC)

Cat. Num. AAA6290607

• Gene Names: CYTH4; CYT4; PSCD4; DJ63G5.1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CYTH4; Polyclonal Antibody; CT (CYTH4; CYT4; PSCD4; Cytohesin-4; PH; SEC7 and coiled-coil domain-containing protein 4) (APC); SEC7 and coiled-coil domain-containing protein 4; anti-CYTH4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CYTH4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CYTH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-319 amino acids from the C-terminal region of human CYTH4.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CYTH4 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the CYTH4 antibody detected the CYTH4 protein (arrow).)

Related Product Information for anti-CYTH4 antibody

The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with both ARF1 and ARF5 but is inactive with ARF6. The structures of this gene and CYTH1 are very similar. [provided by RefSeq].

Product Categories/Family for anti-CYTH4 antibody

Antibodies; CCDC (Coiled-coil Domain-containing)

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Morishige, M., et al. Nat. Cell Biol. 10(1):85-92(2008). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004). Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 7019505
• NCBI GeneID: 27128
• NCBI Accession #: NP_037517.1
• NCBI GenBank Nucleotide #: NM_013385.4
• UniProt Accession #: Q9UIA0; Q5R3F9; Q9UGT6
• Molecular Weight: 45,672 Da
• NCBI Official Full Name: cytohesin-4 isoform 1
• NCBI Official Synonym Full Names: cytohesin 4
• NCBI Official Symbol: CYTH4
• NCBI Official Synonym Symbols: CYT4; PSCD4; DJ63G5.1
• NCBI Protein Information: cytohesin-4
• UniProt Protein Name: Cytohesin-4
• Protein Family: Cytohesin
• UniProt Gene Name: CYTH4
• UniProt Synonym Gene Names: CYT4; PSCD4
• UniProt Entry Name: CYH4_HUMAN

NCBI Description

This gene encodes a member of the PSCD family of proteins, which have an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family function as GEPs for ADP-ribosylation factors (ARFs), which are guanine nucleotide-binding proteins involved in vesicular trafficking pathways. This protein exhibits GEP activity in vitro with ARF1 and ARF5, but is inactive with ARF6. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]