Rabbit CYPB Polyclonal Antibody

CYPB (Peptidyl-prolyl cis-trans Isomerase B, PPIase B, CYP-S1, Cyclophilin B, Rotamase B, S-cyclophilin, SCYLP, PPIB)

Cat. Num. AAA644803

• Reactivity: Avian, Bovine, Canine, Equine, Human, Mouse, Porcine
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: CYPB; Polyclonal Antibody; CYPB (Peptidyl-prolyl cis-trans Isomerase B; PPIase B; CYP-S1; Cyclophilin B; Rotamase B; S-cyclophilin; SCYLP; PPIB); Anti -CYPB (Peptidyl-prolyl cis-trans Isomerase B; anti-CYPB antibody

• Host: Rabbit
• Reactivity: Avian, Bovine, Canine, Equine, Human, Mouse, Porcine
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Cyclophilin B. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

• Applicable Applications for anti-CYPB antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5-2ug/ml
• Immunogen: Synthetic peptide corresponding to aa100-200 of human Cyclophilin B.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Cyclophilin B in A) HeLa cell lysate and B) 293 cell lysate using at 0.5 and 2ug/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.)

Related Product Information for anti-CYPB antibody

Peptidylprolyl isomerase B, also known as Cyclophilin B and PPIB, is a 216aa containing enzyme with peptidyl-prolyl cis-trans isomerase (PPIase) activity and is associated with the secretory pathways and released in biological fluids. It belongs to the cyclophilin-type PPIase family and PPIase B subfamily with a PPIase cyclophilin-type domain and is usually located in the lumen of endoplasmic reticulum and melanosomes. PPIases accelerate the folding of proteins and are known to catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. This cyclosporine-binding protein can also bind to cells derived from T- and B-lymphocytes, and is inhibited by the immunosuppressive drug cyclosporin A (CsA) and is hence an important element in the study of CsA-mediated immunosuppression. Reports also suggest that PPIB is an important component of the HCV replication machinery, acting as a stimulatory regulator of NS5B and could be a possible target for antiviral therapy.

Product Categories/Family for anti-CYPB antibody

Antibodies; Abs to Cyclophilin

NCBI and Uniprot Product Information

• NCBI GI #: 2618851
• NCBI Official Full Name: CypB
• Protein Family: Bifunctional cytochrome P450/NADPH--P450 reductase

Uniprot Description

Involved in the biosynthesis of pulcherrimin, a red extracellular pigment. Catalyzes the oxidation of cyclo(L-Leu-L-Leu) (cLL) to yield pulcherriminic acid which forms pulcherrimin via a nonenzymic reaction with Fe3+. Substrates with small alkyl groups (cAA, cLG, cLP) exhibit weaker binding to CYP134A1, but substrates with larger hydrophobic side chains bind in a similar regime to cLL.