Rabbit CYP4F12 Polyclonal Antibody | anti-CYP4F12 antibody

CYP4F12 Polyclonal Antibody

Cat. Num. AAA9128856

• Gene Names: CYP4F12; CYPIVF12; F22329_1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: CYP4F12; Polyclonal Antibody; CYP4F12 Polyclonal Antibody; anti-CYP4F12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 524

• Applicable Applications for anti-CYP4F12 antibody: Western Blot (WB)
• Application Notes: WB 1:500 - 1:2000
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human CYP4F12
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CYP4F12 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Related Product Information for anti-CYP4F12 antibody

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein likely localizes to the endoplasmic reticulum. When expressed in yeast the enzyme is capable of oxdizing arachidonic acid. It can also catalyze the epoxidation of 22:6n-3 and 22:5n-3 polyunsaturated long-chain fatty acids. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 333033782
• NCBI GeneID: 66002
• NCBI Accession #: NP_076433.3
• NCBI GenBank Nucleotide #: NM_023944.3
• UniProt Accession #: Q9HCS2; O60389; Q5JPJ7; Q9HCS1; E7ET51
• Molecular Weight: 60kDa
• NCBI Official Full Name: cytochrome P450 4F12
• NCBI Official Synonym Full Names: cytochrome P450, family 4, subfamily F, polypeptide 12
• NCBI Official Symbol: CYP4F12
• NCBI Official Synonym Symbols: CYPIVF12; F22329_1
• NCBI Protein Information: cytochrome P450 4F12
• UniProt Protein Name: Cytochrome P450 4F12
• Protein Family: Cytochrome
• UniProt Gene Name: CYP4F12
• UniProt Entry Name: CP4FC_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein likely localizes to the endoplasmic reticulum. When expressed in yeast the enzyme is capable of oxdizing arachidonic acid. It can also catalyze the epoxidation of 22:6n-3 and 22:5n-3 polyunsaturated long-chain fatty acids. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

CYP4F12: Catalyzes leukotriene B4 omega-hydroxylation and arachidonic acid omega-hydroxylation but with an activity much lower than that of CYP4F2. Catalyzes the hydroxylation of the antihistamine ebastine. Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; Membrane protein, multi-pass; EC 1.14.13.30; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; apical plasma membrane; cytoplasm; integral to membrane

Molecular Function: leukotriene-B4 20-monooxygenase activity; iron ion binding; heme binding; arachidonic acid epoxygenase activity; vitamin-K-epoxide reductase (warfarin-sensitive) activity; alkane 1-monooxygenase activity

Biological Process: vitamin E metabolic process; vitamin K biosynthetic process; very-long-chain fatty acid metabolic process; xenobiotic metabolic process; renal water homeostasis; negative regulation of blood coagulation; arachidonic acid metabolic process; epoxygenase P450 pathway; sodium ion homeostasis; long-chain fatty acid metabolic process; drug metabolic process; pressure natriuresis