Rabbit anti-Human CYP3A4 Polyclonal Antibody | anti-CYP3A4 antibody

CYP3A4, ID (Cytochrome P450 3A4, Quinine 3-monooxygenase, CYPIIIA4, Nifedipine Oxidase, Cytochrome P450 3A3, CYPIIIA3, Cytochrome P450 HLp, Taurochenodeoxycholate 6-alpha-hydroxylase, Cytochrome P450 NF-25, Cytochrome P450-PCN1, Albendazole Monooxygenase,

Cat. Num. AAA6471501

• Gene Names: CYP3A4; HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CYP3A4; Polyclonal Antibody; ID (Cytochrome P450 3A4; Quinine 3-monooxygenase; CYPIIIA4; Nifedipine Oxidase; Cytochrome P450 3A3; CYPIIIA3; Cytochrome P450 HLp; Taurochenodeoxycholate 6-alpha-hydroxylase; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Albendazole Monooxygenase; ; anti-CYP3A4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYP3A4.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CYP3A4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CYP3A4 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CYP3A4 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). CYP3A4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CYP3A4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP3A4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CYP3A4 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CYP3A4 antibody

Antibodies; Enzymes; Cytochrome

References

1. Sandanaraj, E., Clin. Cancer Res. 14(21), 7116-7126 (2008). 2. Nelson, D.R., Pharmacogenetics 14(1), 1-18 (2004). 3. Inoue, K., Jpn. J. Hum. Genet. 37(2), 133-138 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 13435386
• NCBI GeneID: 1576
• NCBI Accession #: NP_059488
• NCBI GenBank Nucleotide #: NM_017460
• UniProt Accession #: P08684
• Molecular Weight: 57kDa
• NCBI Official Full Name: cytochrome P450 3A4 isoform 1
• NCBI Official Synonym Full Names: cytochrome P450 family 3 subfamily A member 4
• NCBI Official Symbol: CYP3A4
• NCBI Official Synonym Symbols: HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1
• NCBI Protein Information: cytochrome P450 3A4
• UniProt Protein Name: Cytochrome P450 3A4
• Protein Family: Cytochrome
• UniProt Gene Name: CYP3A4
• UniProt Synonym Gene Names: CYP3A3
• UniProt Entry Name: CP3A4_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2011]

Uniprot Description

CYP3A4: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2- exo-monooxygenase. The enzyme also hydroxylates etoposide. Belongs to the cytochrome P450 family.

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Membrane protein, integral; Cell surface; Xenobiotic Metabolism - drug metabolism - other enzymes; Xenobiotic Metabolism - metabolism by cytochrome P450; Oxidoreductase; Lipid Metabolism - linoleic acid; EC 1.14.14.1; Cofactor and Vitamin Metabolism - retinol

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm; integral to membrane

Molecular Function: enzyme binding; quinine 3-monooxygenase activity; iron ion binding; taurochenodeoxycholate 6alpha-hydroxylase activity; albendazole monooxygenase activity; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; steroid hydroxylase activity; oxidoreductase activity; oxygen binding; monooxygenase activity; testosterone 6-beta-hydroxylase activity; steroid binding; vitamin D3 25-hydroxylase activity

Biological Process: steroid metabolic process; drug catabolic process; xenobiotic metabolic process; exogenous drug catabolic process; androgen metabolic process; monoterpenoid metabolic process; alkaloid catabolic process; lipid metabolic process; heterocycle metabolic process; drug metabolic process; steroid catabolic process; vitamin D metabolic process