Rabbit anti-Human CYP2A7 Polyclonal Antibody | anti-CYP2A7 antibody

CYP2A7, ID (CYP2A7, Cytochrome P450 2A7, CYPIIA7, Cytochrome P450 IIA4) (PE)

Cat. Num. AAA6290484

• Gene Names: CYP2A7; CPA7; CPAD; CYP2A; CYPIIA7; P450-IIA4
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CYP2A7; Polyclonal Antibody; ID (CYP2A7; Cytochrome P450 2A7; CYPIIA7; Cytochrome P450 IIA4) (PE); Cytochrome P450 IIA4; anti-CYP2A7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CYP2A7 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CYP2A7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-156 amino acids from the Central region of human CYP2A7.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CYP2A7 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CYP2A7 antibody detected the CYP2A7 protein (arrow).)

Immunohistochemistry (IHC)

(CYP2A7 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CYP2A7 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CYP2A7 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CYP2A7 antibody

CYP2A7 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum; its substrate has not yet been determined. CYP2A7, which produces two transcript variants, is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.

Product Categories/Family for anti-CYP2A7 antibody

Antibodies; Enzymes; Cytochrome

References

Fukami, T., et al. Pharmacogenomics J. 6(6):401-412(2006). Nelson, D.R., et al. Pharmacogenetics 14(1):1-18(2004). Oscarson, M., et al. Hum. Mutat. 20(4):275-283(2002). Godoy, W., et al. Carcinogenesis 23(4):611-616(2002). Koskela, S., et al. Biochem. Pharmacol. 57(12):1407-1413(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 15147328
• NCBI GeneID: 1549
• NCBI Accession #: NP_085079.2
• NCBI GenBank Nucleotide #: NM_030589.2
• UniProt Accession #: P20853; Q13121
• Molecular Weight: 56,425 Da
• NCBI Official Full Name: cytochrome P450 2A7 isoform 2
• NCBI Official Synonym Full Names: cytochrome P450, family 2, subfamily A, polypeptide 7
• NCBI Official Symbol: CYP2A7
• NCBI Official Synonym Symbols: CPA7; CPAD; CYP2A; CYPIIA7; P450-IIA4
• NCBI Protein Information: cytochrome P450 2A7; cytochrome P450IIA4; cytochrome P450 IIA4; cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 7
• UniProt Protein Name: Cytochrome P450 2A7
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2A7
• UniProt Entry Name: CP2A7_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum; its substrate has not yet been determined. This gene, which produces two transcript variants, is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP2A7: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family.

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 1.14.14.1; Endoplasmic reticulum; Secondary Metabolites Metabolism - caffeine

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxygen binding

Biological Process: coumarin metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; epoxygenase P450 pathway