Rabbit anti-Human CYP20A1 Polyclonal Antibody | anti-CYP20A1 antibody

CYP20A1, ID (CYP20A1, Cytochrome P450 20A1) (PE)

Cat. Num. AAA6290429

• Gene Names: CYP20A1; CYP-M
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CYP20A1; Polyclonal Antibody; ID (CYP20A1; Cytochrome P450 20A1) (PE); Cytochrome P450 20A1; anti-CYP20A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CYP20A1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: CYP20A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-258 amino acids from the Central region of human CYP20A1.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CYP20A1 (arrow) using rabbit polyclonal CYP20A1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYP20A1 gene.)

Flow Cytometry (FC/FACS)

(CYP20A1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CYP20A1 antibody

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein lacks one amino acid of the conserved heme binding site. It also lacks the conserved I-helix motif AGX(D,E)T, suggesting that its substrate may carry its own oxygen. [provided by RefSeq].

Product Categories/Family for anti-CYP20A1 antibody

Antibodies; Enzymes; Cytochrome

References

Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009). Jiang, J.H., et al. Ai Zheng 23(6):672-677(2004). Nelson, D.R., et al. Pharmacogenetics 14(1):1-18(2004). Werck-Reichhart, D., et al. Genome Biol. 1 (6), REVIEWS3003 (2000): Smith, G., et al. Xenobiotica 28(12):1129-1165(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 29171730
• NCBI GeneID: 57404
• NCBI Accession #: NP_803882.1
• NCBI GenBank Nucleotide #: NM_177538.2
• UniProt Accession #: Q6UW02; Q4ZG61; Q8N4Q8; Q8WWA9
• Molecular Weight: 52,432 Da
• NCBI Official Full Name: cytochrome P450 20A1
• NCBI Official Synonym Full Names: cytochrome P450, family 20, subfamily A, polypeptide 1
• NCBI Official Symbol: CYP20A1
• NCBI Official Synonym Symbols: CYP-M
• NCBI Protein Information: cytochrome P450 20A1; cytochrome P450 monooxygenase
• UniProt Protein Name: Cytochrome P450 20A1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP20A1
• UniProt Entry Name: CP20A_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein lacks one amino acid of the conserved heme binding site. It also lacks the conserved I-helix motif AGX(D,E)T, suggesting that its substrate may carry its own oxygen. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP20A1: a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein lacks one amino acid of the conserved heme binding site. It also lacks the conserved I-helix motif AGX(D,E)T, suggesting that its substrate may carry its own oxygen. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, integral; EC 1.14.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 2q33.2

Cellular Component: membrane; integral to membrane

Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; iron ion binding; heme binding; monooxygenase activity