Rabbit anti-Human CYP17A1 Polyclonal Antibody | anti-CYP17A1 antibody

CYP17A1, ID (Steroid 17-alpha-hydroxylase/17,20 Lyase, Steroid 17-alpha-Monooxygenase, Cytochrome P450-C17, Cytochrome P450c17, CYPXVII, Cytochrome P450 17A1, Cytochrome P450-C17, Cytochrome P450c17, CYP17, S17AH) (HRP)

Cat. Num. AAA6471288

• Gene Names: CYP17A1; CPT7; CYP17; S17AH; P450C17
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CYP17A1; Polyclonal Antibody; ID (Steroid 17-alpha-hydroxylase/17; 20 Lyase; Steroid 17-alpha-Monooxygenase; Cytochrome P450-C17; Cytochrome P450c17; CYPXVII; Cytochrome P450 17A1; CYP17; S17AH) (HRP); anti-CYP17A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYP17A1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CYP17A1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CYP17A1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CYP17A1 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Western Blot (WB)

(Western blot analysis of anti-CYP17A1 Antibody (Center) in K562 cell line lysates (35ug/lane). CYP17A1(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-CYP17A1 antibody

Antibodies; Enzymes; Cytochrome

References

1. Yuan, X., Cancer Epidemiol. Biomarkers Prev. 17(12), 3621-3627 (2008). 2. Nelson, D.R., Pharmacogenetics 14(1), 1-18 (2004). 3. Imai, T., Hum. Genet. 89(1), 95-96 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 4503195
• NCBI GeneID: 1586
• NCBI Accession #: NP_000093
• NCBI GenBank Nucleotide #: NM_000102
• UniProt Accession #: P05093
• Molecular Weight: 57,371 Da
• NCBI Official Full Name: steroid 17-alpha-hydroxylase/17,20 lyase
• NCBI Official Synonym Full Names: cytochrome P450, family 17, subfamily A, polypeptide 1
• NCBI Official Symbol: CYP17A1
• NCBI Official Synonym Symbols: CPT7; CYP17; S17AH; P450C17
• NCBI Protein Information: steroid 17-alpha-hydroxylase/17,20 lyase; 17-alpha-hydroxyprogesterone aldolase; CYPXVII; cytochrome P450 17A1; cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia; cytochrome P450-C17; cytochrome P450c17; cytochrome p450 X
• UniProt Protein Name: Steroid 17-alpha-hydroxylase/17,20 lyase
• Protein Family: Steroid 17-alpha-hydroxylase/17,20 lyase
• UniProt Gene Name: CYP17A1
• UniProt Synonym Gene Names: CYP17; S17AH; Cytochrome P450c17
• UniProt Entry Name: CP17A_HUMAN

Uniprot Description

CYP17A1: Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5). AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: salt wasting (SW, the most severe type), simple virilizing (SV, less severely affected patients), with normal aldosterone biosynthesis, non-classic form or late onset (NC or LOAH), and cryptic (asymptomatic). Belongs to the cytochrome P450 family.

Protein type: EC 4.1.2.30; EC 1.14.99.9; Oxidoreductase; Lipid Metabolism - C21-steroid hormone

Chromosomal Location of Human Ortholog: 10q24.3

Cellular Component: endoplasmic reticulum membrane; cell soma; mitochondrion; endoplasmic reticulum; axon

Molecular Function: 17-alpha-hydroxyprogesterone aldolase activity; steroid 17-alpha-monooxygenase activity; iron ion binding; heme binding; oxygen binding

Biological Process: steroid metabolic process; response to cAMP; dibenzo-p-dioxin metabolic process; androgen biosynthetic process; progesterone metabolic process; response to insecticide; Leydig cell differentiation; biphenyl metabolic process; response to methylmercury; hormone biosynthetic process; response to drug; response to nutrient levels; response to retinoic acid; adrenal gland development; phthalate metabolic process; hippocampus development; response to herbicide; ovulation; response to acetate; response to steroid hormone stimulus; xenobiotic metabolic process; response to cytokine stimulus; response to ionizing radiation; glucocorticoid biosynthetic process; sex differentiation; sterol metabolic process; steroid biosynthetic process; phenol metabolic process

Disease: Adrenal Hyperplasia, Congenital, Due To 17-alpha-hydroxylase Deficiency