Rabbit CYLD Polyclonal Antibody | anti-CYLD antibody

CYLD (Cylindromatosis, EAC, MFT, SBS, TEM, BRSS, CDMT, MFT1, CYLD1, CYLDI, USPL2) (Biotin)

Cat. Num. AAA6413375

• Gene Names: CYLD; EAC; MFT; SBS; TEM; BRSS; CDMT; MFT1; CYLD1; CYLDI; USPL2
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: CYLD; Polyclonal Antibody; CYLD (Cylindromatosis; EAC; MFT; SBS; TEM; BRSS; CDMT; MFT1; CYLD1; CYLDI; USPL2) (Biotin); anti-CYLD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes CYLD. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-CYLD antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:200-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human CYLD.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Categories/Family for anti-CYLD antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 51316104
• NCBI GeneID: 1540
• UniProt Accession #: Q9NQC7; O94934; Q7L3N6; Q96EH0; Q9NZX9
• Molecular Weight: 956
• NCBI Official Full Name: Ubiquitin carboxyl-terminal hydrolase CYLD
• NCBI Official Synonym Full Names: cylindromatosis (turban tumor syndrome)
• NCBI Official Symbol: CYLD
• NCBI Official Synonym Symbols: EAC; MFT; SBS; TEM; BRSS; CDMT; MFT1; CYLD1; CYLDI; USPL2
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin thioesterase CYLD; deubiquitinating enzyme CYLD; ubiquitin thiolesterase CYLD; ubiquitin specific peptidase like 2; ubiquitin-specific-processing protease CYLD; probable ubiquitin carboxyl-terminal hyd
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase CYLD
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: CYLD
• UniProt Synonym Gene Names: CYLD1; KIAA0849
• UniProt Entry Name: CYLD_HUMAN

NCBI Description

This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

CYLD: Protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Has endodeubiquitinase activity. Plays an important role in the regulation of pathways leading to NF-kappa-B activation. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. Required for normal cell cycle progress and normal cytokinesis. Inhibits nuclear translocation of NF-kappa-B. Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. Negatively regulates TNFRSF11A signaling and osteoclastogenesis. Interacts (via CAP-Gly domain) with IKBKG/NEMO (via proline-rich C-terminal region). Interacts with TRAF2 and TRIP. Interacts with PLK1, DVL1, DVL3, MAVS, TBK1, IKKE and DDX58. Interacts (via CAP-Gly domain) with microtubules. Interacts with HDAC6 and BCL3. Interacts with SQSTM1 and MAP3K7. Identified in a complex with TRAF6 and SQSTM1. Detected in fetal brain, testis, and skeletal muscle, and at a lower level in adult brain, leukocytes, liver, heart, kidney, spleen, ovary and lung. Isoform 2 is found in all tissues except kidney. Inhibited by phosphorylation at serine residues. Belongs to the peptidase C67 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; Protease; Tumor suppressor; EC 3.4.19.12; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: nucleoplasm; centrosome; extrinsic to internal side of plasma membrane; perinuclear region of cytoplasm; cytoplasmic microtubule; spindle; midbody; cytosol

Molecular Function: protein binding; structural constituent of ribosome; zinc ion binding; ubiquitin-specific protease activity; protein kinase binding

Biological Process: ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway; inhibition of NF-kappaB transcription factor; regulation of mitotic cell cycle; innate immune response; negative regulation of NF-kappaB import into nucleus; cell cycle; negative regulation of interferon type I production; negative regulation of T cell differentiation

Disease: Brooke-spiegler Syndrome; Cylindromatosis, Familial; Trichoepithelioma, Multiple Familial, 1