Rabbit cyclin E Polyclonal Antibody | anti-Ccne1 antibody

cyclin E (C19) Antibody

Cat. Num. AAA440015

• Gene Names: Ccne1; Ccne; CYCLE
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Synonyms: cyclin E; Polyclonal Antibody; cyclin E (C19) Antibody; anti-Ccne1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Cyclin E (C19) MBS440015 reacts with cyclin E of mouse, rat, and human origin
• Form/Format: Each vial contains 200 ug/ml of affinity purified rabbit IgG, cyclin E (C19) MBS440015, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.

• Applicable Applications for anti-Ccne1 antibody: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: WB: 1:200
• Origin: Cyclin E (C19) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminus of rat cyclin E.
• Competition Studies: A blocking peptide is also available, MBS443015, for use in competition studies. Each vial contains 100 ug of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 ug BSA.
• Preparation and Storage: Store this product at 4º C, do not freeze.

Related Product Information for anti-Ccne1 antibody

During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks (1,2). Early in the G1 phase of the cell cycle cyclin D1 induction is followed by cyclin E induction (3,4). This sequential progression is marked early on in G1by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB (3,5). The final transition into S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2 (5,6).

References

1.) Song SY, Lee I, Park C, Lee H, Hahm JC, Kang WK. 2005. Neolignans from Saururus chinensis inhibit PC-3 prostate cancer cell growth via apoptosis and senescence-like mechanisms. Int J Mol Med. Oct;16(4):517-23.

1. Bandara LR, Adamczewski JP, Zamanian M, Poon RY, Hunt T, Thangue NB. 1992. Cyclin A recruits p33cdk2 to the cellular transcription factor DRTF1. J Cell Sci Suppl 16: 77-85.2. Desdouets C, Sobczak-Thepot J, Murphy M, Brechot C. 1995. Cyclin A: Function and expression during cell proliferation. Prog Cell Cycle Res 1:115-123.3. Musgrove EA, Sarcevic B, Sutherland RL. 1996. Inducible expression of cyclin D1 in T-47D human breast cancer cells is sufficient for Cdk2 activation and pRB hyperphosphorylation. J Cell Biochem 60(3): 363-378.4. Geng Y, Whorishey W, Park MY, Bronson RT, Medema RH, Li T, Weinberg RA, Sicinski P. 1999. Rescue of cyclin D1 deficiency by knockin cyclin E. Cell 97(6): 767-777.5. Prall OW, Sarcevic B, Musgrove EA, Watts CK, Sutherland RL. 1997. Estrogen-induced activation of Cdk4 and Cdk2 during G1-S phase progression is accompanied by increased cyclin D1 expression and decreased cyclin-dependent kinase inhibitor association with cyclin E-Cdk2. J Biol Chem 272(16): 10882-10894.6. Sauer K, Lehner CF. 1995. The role of cyclin E in the regulation of entry into S phase. Prog Cell Cycle Res 1: 125-139.

NCBI and Uniprot Product Information

• NCBI GI #: 158187539
• NCBI GeneID: 25729
• NCBI Accession #: NP_001094291.1
• NCBI GenBank Nucleotide #: NM_001100821.1
• UniProt Accession #: P39949; O09138
• NCBI Official Full Name: G1/S-specific cyclin-E1
• NCBI Official Synonym Full Names: cyclin E1
• NCBI Official Symbol: Ccne1
• NCBI Official Synonym Symbols: Ccne; CYCLE
• NCBI Protein Information: G1/S-specific cyclin-E1; cyclin E
• UniProt Protein Name: G1/S-specific cyclin-E1
• UniProt Gene Name: Ccne1
• UniProt Synonym Gene Names: Ccne
• UniProt Entry Name: CCNE1_RAT

NCBI Description

a cyclin-like protein; may be involved in growth stimulation [RGD, Feb 2006]

Uniprot Description

CCNE1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Two alternatively spliced isoforms have been described.

Protein type: Activator; Cell cycle regulation; Nuclear receptor co-regulator

Cellular Component: centrosome; cyclin-dependent protein kinase holoenzyme complex; cytoplasm; nucleoplasm; nucleus

Molecular Function: cyclin-dependent protein kinase regulator activity; kinase activity; protein complex binding; protein kinase binding

Biological Process: antral ovarian follicle growth; cellular response to nutrient; DNA replication initiation; liver development; negative regulation of transcription from RNA polymerase II promoter; organ regeneration; positive regulation of cell differentiation; protein amino acid phosphorylation; regulation of cell cycle; response to corticosterone stimulus; response to cytokine stimulus; response to drug; response to estradiol stimulus; response to ethanol; response to methylmercury; response to organic cyclic substance; response to organic nitrogen; response to progesterone stimulus; response to purine; response to steroid hormone stimulus; response to vitamin E; synapsis; telomere maintenance; Wnt receptor signaling pathway