Rabbit anti-Human CYBA Polyclonal Antibody | anti-CYBA antibody

CYBA, CT (CYBA, Cytochrome b-245 light chain, Cytochrome b(558) alpha chain, Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22kD polypeptide, Superoxide-generating NADPH oxidase light chain subunit, p22 phagocyte B-cytochrome, p22-phox) (FITC)

Cat. Num. AAA6290334

• Gene Names: CYBA; p22-PHOX
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CYBA; Polyclonal Antibody; CT (CYBA; Cytochrome b-245 light chain; Cytochrome b(558) alpha chain; Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22kD polypeptide; Superoxide-generating NADPH oxidase light chain subunit; p22 phagocyte B-cytochrome; p22-phox) (FITC); p22-phox; anti-CYBA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CYBA antibody: Immunofluorescence (IF), FLISA, Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CYBA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-153 amino acids from the C-terminal region of human CYBA.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CYBA Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the CYBA antibody detected the CYBA protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CYBA Antibody (C-term) with NCI-H292 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-CYBA antibody

Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells.

Product Categories/Family for anti-CYBA antibody

Antibodies; Enzymes; Cytochrome

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Katakami, N., et al. Atherosclerosis 212(2):534-538(2010). Tu, Y.C., et al. Acta Pharmacol. Sin. 31(7):874-880(2010). Moreno, M.U., et al. Drug News Perspect. 23(5):316-324(2010). Cross, D.S., et al. BMC Genet. 11, 51 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 68509914
• NCBI GeneID: 1535
• NCBI Accession #: NP_000092.2
• NCBI GenBank Nucleotide #: NM_000101.3
• UniProt Accession #: P13498; Q14090; Q9BR72
• Molecular Weight: 21,013 Da
• NCBI Official Full Name: cytochrome b-245 light chain
• NCBI Official Synonym Full Names: cytochrome b-245, alpha polypeptide
• NCBI Official Symbol: CYBA
• NCBI Official Synonym Symbols: p22-PHOX
• NCBI Protein Information: cytochrome b-245 light chain; cytochrome b light chain; cytochrome b(558) alpha chain; cytochrome b(558) alpha-subunit; cytochrome b, alpha polypeptide; cytochrome b558 subunit alpha; flavocytochrome b-558 alpha polypeptide; neutrophil cytochrome b 22 kDa
• UniProt Protein Name: Cytochrome b-245 light chain
• Protein Family: Cytochrome
• UniProt Gene Name: CYBA
• UniProt Synonym Gene Names: p22phox
• UniProt Entry Name: CY24A_HUMAN

NCBI Description

Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells. [provided by RefSeq, Jul 2008]

Uniprot Description

CYBA: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide. Defects in CYBA are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-negative (ARCGD). Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. Belongs to the p22phox family.

Protein type: Oxidoreductase; Membrane protein, integral; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 16q24

Cellular Component: Golgi apparatus; phagocytic vesicle membrane; membrane; cell soma; mitochondrion; apical plasma membrane; dendrite; plasma membrane; endosome; secretory granule; NADPH oxidase complex

Molecular Function: protein binding; electron carrier activity; protein heterodimerization activity; metal ion binding; superoxide-generating NADPH oxidase activity; heme binding; SH3 domain binding

Biological Process: response to drug; respiratory burst; response to nutrient levels; interaction with host; angiotensin-mediated regulation of glomerular filtration; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of cell growth; regulation of release of sequestered calcium ion into cytosol; antigen processing and presentation of peptide antigen via MHC class I; cytochrome complex assembly; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; smooth muscle hypertrophy; positive regulation of endothelial cell proliferation; inflammatory response; vascular endothelial growth factor receptor signaling pathway; superoxide release; hydrogen peroxide biosynthetic process

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-negative