Western Blot (WB)
((0.1ug/ml) staining of Mouse Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat CYB5R3/Dia 1 Polyclonal Antibody | anti-CYB5R3 antibody
Goat anti-CYB5R3/Dia 1 (mouse) Antibody
Western Blot: Approx 35kDa band observed in Mouse Liver lysates as well as in mouse and rat brain lysates (calculated MW of 34.1kDa according to mouse NP_084063.1). Recommended concentration: 0.1-1ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of Mouse Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Mouse Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
Uniprot Description
CYB5R3: Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Defects in CYB5R3 are the cause of methemoglobinemia CYB5R3-related (METHB-CYB5R3). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. There are two types of methemoglobinemia CYB5R3-related. In type 1, the defect affects the soluble form of the enzyme, is restricted to red blood cells, and causes well- tolerated methemoglobinemia. In type 2, the defect affects both the soluble and microsomal forms of the enzyme and is thus generalized, affecting red cells, leukocytes and all body tissues. Type 2 methemoglobinemia is associated with mental deficiency and other neurologic symptoms. Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. 3 isoforms of the human protein are produced by alternative promoter.
Protein type: EC 1.6.2.2; Oxidoreductase; Mitochondrial; Carbohydrate Metabolism - amino sugar and nucleotide sugar
Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; membrane; mitochondrial inner membrane; mitochondrial outer membrane; mitochondrion
Molecular Function: ADP binding; AMP binding; cytochrome-b5 reductase activity; FAD binding; NAD binding; oxidoreductase activity; protein binding
Biological Process: cholesterol biosynthetic process; cholesterol metabolic process; lipid metabolic process; steroid biosynthetic process; steroid metabolic process; sterol biosynthetic process
Research Articles on CYB5R3
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Product Notes
The CYB5R3 cyb5r3 (Catalog #AAA421792) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CYB5R3/Dia 1 (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CYB5R3/Dia 1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 35kDa band observed in Mouse Liver lysates as well as in mouse and rat brain lysates (calculated MW of 34.1kDa according to mouse NP_084063.1). Recommended concentration: 0.1-1ug/ml. Researchers should empirically determine the suitability of the CYB5R3 cyb5r3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PNLERVGHPK ERC. It is sometimes possible for the material contained within the vial of "CYB5R3/Dia 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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