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Western Blot (WB)
(Western blot analysis in mouse testis tissue lysates (35ug/lane) usingMBS6002889. CXXC4 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse CXXC4 Polyclonal Antibody | anti-CXXC4 antibody
CXXC4, CT (CXXC4, IDAX, CXXC-type zinc finger protein 4, Inhibition of the Dvl and axin complex protein) (MaxLight 490)
Gene Names
CXXC4; IDAX
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
CXXC4; Polyclonal Antibody; CT (CXXC4; IDAX; CXXC-type zinc finger protein 4; Inhibition of the Dvl and axin complex protein) (MaxLight 490); Inhibition of the Dvl and axin complex protein; anti-CXXC4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CXXC4. Species Crossreactivity: mouse
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-CXXC4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), FLISA
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 158-185 in the C-terminal region of human CXXC4, UniProt Accession #Q9H2H0; Q9EQC9; Q6NXI8. Species sequence homology: rat
Conjugate
MaxLight490
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis in mouse testis tissue lysates (35ug/lane) usingMBS6002889. CXXC4 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis in mouse testis tissue lysates (35ug/lane) usingMBS6002889. CXXC4 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6002889. This demonstrates that MBS6002889 detected the CXXC4 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6002889. This demonstrates that MBS6002889 detected the CXXC4 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry in formalin-fixed and paraffin-embedded human colon carcinoma usingMBS6002889, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6002889 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry in formalin-fixed and paraffin-embedded human colon carcinoma usingMBS6002889, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6002889 for immunohistochemistry.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of K562 cells using MBS6002889 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of K562 cells using MBS6002889 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CXXC4 antibody
CXXC4 acts as a negative regulator of the Wnt signaling pathway via its interaction with DVL1.
Product Categories/Family for anti-CXXC4 antibody
References
1. Kojima, T., et al. Oncogene 28(2):297-305(2009). 2. Hino, S., et al. Mol. Cell. Biol. 21(1):330-342(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,978 Da
NCBI Official Full Name
CXXC-type zinc finger protein 4
NCBI Official Synonym Full Names
CXXC finger protein 4
NCBI Official Symbol
CXXC4
NCBI Official Synonym Symbols
IDAX
NCBI Protein Information
CXXC-type zinc finger protein 4; CXXC finger 4; inhibition of the Dvl and axin complex protein; Dvl-binding protein IDAX (inhibition of the Dvl and Axin complex)
UniProt Protein Name
CXXC-type zinc finger protein 4
Protein Family
UniProt Gene Name
CXXC4
UniProt Synonym Gene Names
IDAX
UniProt Entry Name
CXXC4_HUMAN
Uniprot Description
CXXC4: Acts as a negative regulator of the Wnt signaling pathway via its interaction with DVL1.
Protein type: Inhibitor
Chromosomal Location of Human Ortholog: 4q24
Cellular Component: cytoplasmic membrane-bound vesicle; cytoplasm; cytoplasmic vesicle
Molecular Function: DNA binding; zinc ion binding; PDZ domain binding
Biological Process: zygotic determination of dorsal/ventral axis; negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway
Research Articles on CXXC4
Similar Products
Product Notes
The CXXC4 cxxc4 (Catalog #AAA6290282) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CXXC4, CT (CXXC4, IDAX, CXXC-type zinc finger protein 4, Inhibition of the Dvl and axin complex protein) (MaxLight 490) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CXXC4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CXXC4 cxxc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXXC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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