Rabbit anti-Human, Rat CXCR3 Polyclonal Antibody | anti-CXCR3 antibody

Anti-CXCR3 Antibody

Cat. Num. AAA176866

• Gene Names: CXCR3; GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: CXCR3; Polyclonal Antibody; Anti-CXCR3 Antibody; CD183 antigen; CD183; chemokine (C-X-C motif) receptor 3; chemokine (C-X-C) receptor 3; CKR-L2IP-10 receptor; CMKAR3; C-X-C chemokine receptor type 3; CXC-R3; CXCR-3; G protein-coupled receptor 9CD182; GPR9; GPR9Mig-R; Interferon-inducible protein 10 receptor; IP10 receptor; IP10-R; Mig receptor; MigR CXCR3 CD182; CKR-L2; Mig-R; MigR C-X-C motif chemokine receptor 3 C-X-C chemokine receptor type 3|G protein-coupled receptor 9|IP-10 receptor|Mig receptor|chemokine (C-X-C motif) receptor 3|chemokine receptor 3|interferon-inducible protein 10 receptor; anti-CXCR3 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: No cross reactivity with other proteins
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 368

• Applicable Applications for anti-CXCR3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: WB: 0.1-0.5ug/ml; Human; IHC (Paraffin-embedded Section): 0.5-1ug/ml; Human, Rat; By Heat; WB: The detection limit for CXCR3 is approximately 0.25ng/lane under reducing conditions.; Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.; Other applications have not been tested. Optimal dilutions should be determined by end users.
• Immunogen: E Coli-derived human CXCR3 recombinant protein (Position: M1-L368). Human CXCR3 shares 86% amino acid (aa) sequence identity with both mouse and rat CXCR3.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.
• Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Our lab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CXCR3 Picoband antibody, MBS176866-1.JPGIHC(P): Rat Kidney Tissue )

Immunohistochemistry (IHC)

(Anti-CXCR3 Picoband antibody, MBS176866-2.JPGIHC(P): Human Tonsil Tissue )

Immunohistochemistry (IHC)

(Anti-CXCR3 Picoband antibody, MBS176866-3.JPGIHC(P): Human Lung Cancer Tissue )

Related Product Information for anti-CXCR3 antibody

Background: Chemokine receptor CXCR3 is a Galphai protein-coupled receptor in the CXC chemokine receptor family. Other names for CXCR3 are G protein-coupled receptor 9 (GPR9) and CD183. It is mapped to Xq13.1. CXCR3 is expressed on malignant B cells from chronic lymphoproliferative disorders, particularly in patients with CLL, and represents a fully functional receptor involved in chemotaxis of malignant B lymphocytes. It is found that in the absence of known etiologic agents, CXCR3 represents a novel target for therapeutic interference early in type 1 diabetes. CXCR3 signaling is associated with MG pathogenesis and proposed that and CXCR3 may serve as novel drug targets to treat MG. CXCR3A and CXCR3B are involved in the chemotactic and vascular effects of CXCL4L1.

NCBI and Uniprot Product Information

• NCBI GI #: 2829400
• NCBI GeneID: 2833
• UniProt Accession #: P49682; O15185; Q7Z710; Q9P2T4; Q9P2T5; B2R982
• NCBI Official Full Name: C-X-C chemokine receptor type 3
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) receptor 3
• NCBI Official Symbol: CXCR3
• NCBI Official Synonym Symbols: GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R
• NCBI Protein Information: C-X-C chemokine receptor type 3; CXC-R3; CXCR-3; Mig receptor; IP10 receptor; IP-10 receptor; G protein-coupled receptor 9; chemokine (C-X-C) receptor 3; interferon-inducible protein 10 receptor
• UniProt Protein Name: C-X-C chemokine receptor type 3
• Protein Family: C-X-C chemokine receptor
• UniProt Gene Name: CXCR3
• UniProt Synonym Gene Names: GPR9; CXC-R3; CXCR-3; IP-10 receptor
• UniProt Entry Name: CXCR3_HUMAN

NCBI Description

This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq, Jun 2011]

Uniprot Description

CXCR3: Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC). Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: C-X-C chemokine binding; chemokine receptor activity; chemokine binding; receptor activity; C-X-C chemokine receptor activity

Biological Process: apoptosis; positive regulation of chemotaxis; calcium-mediated signaling; positive regulation of cAMP metabolic process; chemotaxis; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; negative regulation of angiogenesis; cell surface receptor linked signal transduction; positive regulation of cell proliferation; negative regulation of endothelial cell proliferation; regulation of leukocyte migration; positive regulation of transcription from RNA polymerase II promoter; positive regulation of release of sequestered calcium ion into cytosol; angiogenesis; inflammatory response; cell adhesion; cell motility