Rabbit anti-Human CXCL9 Polyclonal Antibody | anti-CXCL9 antibody

CXCL9, CT (CXCL9, CMK, MIG, SCYB9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Small-inducible cytokine B9) (PE)

Cat. Num. AAA6290220

• Gene Names: CXCL9; CMK; MIG; Humig; SCYB9; crg-10
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CXCL9; Polyclonal Antibody; CT (CXCL9; CMK; MIG; SCYB9; C-X-C motif chemokine 9; Gamma-interferon-induced monokine; Monokine induced by interferon-gamma; Small-inducible cytokine B9) (PE); Small-inducible cytokine B9; anti-CXCL9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CXCL9.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CXCL9 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 96-122 in the C-terminal region of human CXCL9, Swiss/UniProt Accession: Q07325
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB231 cell line lysates (35ug/lane) usingMBS6000657. CXCL9 (arrow) was detected usingMBS6000657.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma using MBS6000657 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6000657 for immunohistochemistry.)

Product Categories/Family for anti-CXCL9 antibody

Antibodies; Interferon

References

1. Gong, J.H., et al. Cell. Immunol. 261(2):105-113(2010). 2. Trotta, T., et al. Mol. Immunol. 47 (2-3), 332-339 (2009). 3. Richards, T.J., et al. Arthritis Rheum. 60(7):2183-2192(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4505187
• NCBI GeneID: 4283
• NCBI Accession #: NP_002407.1
• NCBI GenBank Nucleotide #: NM_002416.2
• UniProt Accession #: Q07325; Q503B4
• Molecular Weight: 14,019 Da
• NCBI Official Full Name: C-X-C motif chemokine 9
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 9
• NCBI Official Symbol: CXCL9
• NCBI Official Synonym Symbols: CMK; MIG; Humig; SCYB9; crg-10
• NCBI Protein Information: C-X-C motif chemokine 9; gamma-interferon-induced monokine; monokine induced by gamma interferon; monokine induced by interferon-gamma; small-inducible cytokine B9
• UniProt Protein Name: C-X-C motif chemokine 9
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: CXCL9
• UniProt Synonym Gene Names: CMK; MIG; SCYB9; HuMIG; MIG
• UniProt Entry Name: CXCL9_HUMAN

NCBI Description

This antimicrobial gene encodes a protein thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL9: Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: protein binding; CXCR3 chemokine receptor binding; chemokine activity; cytokine activity

Biological Process: defense response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; chemotaxis; signal transduction; positive regulation of myoblast differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; cell-cell signaling; cellular defense response; immune response; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; defense response to virus