Rabbit Cullin 2 Polyclonal Antibody | anti-CUL2 antibody

Cullin 2 Polyclonal Antibody

Cat. Num. AAA3012525

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: Cullin 2; Polyclonal Antibody; Cullin 2 Polyclonal Antibody; CUL-2; CUL2; Cul2; CUL 2; cullin 2; cullin homolog 2; Cullin-2; MGC131970; anti-CUL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cullin 2 polyclonal antibody detects endogenous levels of Cullin 2 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 745

• Applicable Applications for anti-CUL2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2,000; IHC: 1:50-1:200; IP: 1:10-1:50
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Cullin 2 on PC-3M and K562 cell lysates using anti-Cullin 2 antibody at 1/1,000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Cullin 2 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-CUL2 antibody

Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex.

NCBI and Uniprot Product Information

• NCBI GI #: 311771637
• NCBI GeneID: 8453
• NCBI Accession #: NP_001185706.1
• NCBI GenBank Nucleotide #: NP_001185706.1
• UniProt Accession #: Q13617
• Molecular Weight: 89kDa
• NCBI Official Full Name: cullin-2 isoform c
• NCBI Official Synonym Full Names: cullin 2
• NCBI Official Symbol: CUL2
• NCBI Protein Information: cullin-2
• UniProt Protein Name: Cullin-2
• Protein Family: Cullin
• UniProt Gene Name: CUL2
• UniProt Synonym Gene Names: CUL-2
• UniProt Entry Name: CUL2_HUMAN

Uniprot Description

CUL2: Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). Belongs to the cullin family.

Protein type: Cell cycle regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 10p11.21

Cellular Component: Cul2-RING ubiquitin ligase complex; nucleoplasm; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of cell proliferation; viral reproduction; protein ubiquitination; cell cycle arrest; G1/S transition of mitotic cell cycle