Rabbit anti-Human, Mouse Cullin 1 / CUL1 Polyclonal Antibody | anti-CUL1 antibody

Rabbit Polyclonal (IgG) to Human Cullin 1 / CUL1

Cat. Num. AAA247945

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: Cullin 1 / CUL1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human Cullin 1 / CUL1; Anti-Cullin 1 / CUL1 Antibody (aa727-776) IHC-plus; CUL1; Cullin 1; Cullin-1; CUL-1; Human Cullin 1; anti-CUL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cullin 1 Antibody detects endogenous levels of total Cullin 1 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 776

• Applicable Applications for anti-CUL1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:20000), IF (1:100 - 1:500), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human Cullin 1 (aa727-776).
• Immunogen: Cullin 1 / CUL1 antibody was raised against synthetic peptide from human Cullin 1 (aa727-776).
• Antigen Modification: aa727-776
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-CUL1 / Cullin 1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of HeLa cells, using Cullin 1 Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from HeLa cells, using Cullin 1 Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-CUL1 antibody

Cullin-1 is a negative regulator of the cell cycle that controls the abundance of G1 cyclins during embryogenesis; Cul1 mutants produce abnormally small cells due to an accelerated progression of the cell cycle G1-to-S-phases and the absence of mitotic arrest phase. Null mutations cause hyperplasia of all tissues. Cul-1 is part of the SCF ubiquitin ligase complex with S KP1 and F box-containing protein. Cul-1 is homologous to the yeast cdc53 protein.

NCBI and Uniprot Product Information

• NCBI GI #: 32307161
• NCBI GeneID: 8454
• NCBI Accession #: NP_003583.2
• NCBI GenBank Nucleotide #: NM_003592.2
• UniProt Accession #: Q13616; O60719; Q08AL6; Q8IYW1; D3DWG3
• Molecular Weight: 89,679 Da
• NCBI Official Full Name: cullin-1
• NCBI Official Synonym Full Names: cullin 1
• NCBI Official Symbol: CUL1
• NCBI Protein Information: cullin-1; CUL-1
• UniProt Protein Name: Cullin-1
• Protein Family: Cullin
• UniProt Gene Name: CUL1
• UniProt Synonym Gene Names: CUL-1
• UniProt Entry Name: CUL1_HUMAN

Uniprot Description

CUL1: a core scaffolding component of multiple cullin-RING-based SCF (SKP1- CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. The cullin subunit serves as a rigid scaffold of SCF complexes. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex depends on the neddylation of the cullin subunit. Interacts with multiple F-box proteins in SCF complexes, directing the ubiquitination of many substrates. These F-box proteins and associated substrates include: BTRC and FBXW11 bind CTNNB1 and participate in Wnt signaling; FBXW11 bind phosphorylated NFKBIA; BTRC binds NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2; SKP2 binds phosphorylated p27kip and is involved in regulation of G1/S transition; SKP2 binds ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1; FBXW7 binds cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1; FBXW2 binds GCM1; FBXO32 binds MYOD1; FBXO7 binds BIRC2 and DLGAP5; FBXO33 binds YBX1; and Cyclin F binds CP110. Interacts with CHEK2, mediating its ubiquitination and regulating its function. Belongs to the cullin family.

Protein type: Ubiquitin conjugating system; Cell cycle regulation

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: nucleoplasm; cullin-RING ubiquitin ligase complex; SCF ubiquitin ligase complex; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; circadian rhythm; protein monoubiquitination; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; viral reproduction; protein ubiquitination; negative regulation of cell proliferation; cell proliferation; organ morphogenesis; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; cell cycle arrest; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle