Rabbit anti-Human, Mouse CUL2 / Cullin 2 Polyclonal Antibody | anti-CUL2 antibody

Rabbit Polyclonal (IgG) to Human CUL2 / Cullin 2

Cat. Num. AAA244259

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CUL2 / Cullin 2; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human CUL2 / Cullin 2; Anti-CUL2 / Cullin 2 Antibody (aa696-745) IHC-plus; CUL2; Cullin homolog 2; CUL-2; Cullin 2; Cullin-2; Human CUL2; anti-CUL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cullin 2 Antibody detects endogenous levels of total Cullin 2 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 745

• Applicable Applications for anti-CUL2 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000), IF (1:100 - 1:500), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human Cullin 2 (aa696-745).
• Immunogen: CUL2 / Cullin 2 antibody was raised against synthetic peptide from human Cullin 2 (aa696-745).
• Antigen Modification: aa696-745
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-CUL2 / Cullin 2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of NIH-3T3 cells, using Cullin 2 Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from LOVO cells, using Cullin 2 Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 19482174
• NCBI GeneID: 8453
• NCBI Accession #: NP_003582.2
• NCBI GenBank Nucleotide #: NM_003591.3
• UniProt Accession #: Q13617; O00200; Q5T2B6; Q9UNF9; B3KT95; B7Z6K8; D3DRY6; G3V1S2
• Molecular Weight: 89,492 Da
• NCBI Official Full Name: cullin-2 isoform c
• NCBI Official Synonym Full Names: cullin 2
• NCBI Official Symbol: CUL2
• NCBI Protein Information: cullin-2; CUL-2
• UniProt Protein Name: Cullin-2
• Protein Family: Cullin
• UniProt Gene Name: CUL2
• UniProt Synonym Gene Names: CUL-2
• UniProt Entry Name: CUL2_HUMAN

Uniprot Description

CUL2: Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). Belongs to the cullin family.

Protein type: Ubiquitin conjugating system; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10p11.21

Cellular Component: nucleoplasm; Cul2-RING ubiquitin ligase complex; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of cell proliferation; viral reproduction; protein ubiquitination; cell cycle arrest; G1/S transition of mitotic cell cycle