Rabbit anti-Human, Mouse CUL5 Polyclonal Antibody | anti-CUL5 antibody

CUL5 Polyclonal Antibody

Cat. Num. AAA3011155

• Gene Names: CUL5; CUL-5; VACM1; VACM-1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CUL5; Polyclonal Antibody; CUL5 Polyclonal Antibody; CUL 5; CUL-5; CUL5_HUMAN; Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1); Cullin-5; Cullin5; VACM 1; VACM-1; VACM1; Vasopressin activated calcium mobilizing receptor 1; Vasopressin activated calcium mobilizing receptor; Vasopressin-activated calcium-mobilizing receptor 1; Vasopressin-activated calcium-mobilizing receptor-1; anti-CUL5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CUL5 polyclonal antibody detects endogenous levels of CUL5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 780

• Applicable Applications for anti-CUL5 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human CUL5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of CUL5 polyclonal antibody)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells, using CUL5 polyclonal antibody)

Related Product Information for anti-CUL5 antibody

Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. Cul-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/Cul-1/F-box protein complex) consists of Skp1 associating with both Cul-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. Cul-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.

NCBI and Uniprot Product Information

• NCBI GI #: 40254446
• NCBI GeneID: 8065
• NCBI Accession #: NP_003469.2
• NCBI GenBank Nucleotide #: NP_003469.2
• UniProt Accession #: Q93034
• Molecular Weight: ~91kDa
• NCBI Official Full Name: cullin-5
• NCBI Official Synonym Full Names: cullin 5
• NCBI Official Symbol: CUL5
• NCBI Official Synonym Symbols: CUL-5; VACM1; VACM-1
• NCBI Protein Information: cullin-5
• UniProt Protein Name: Cullin-5
• Protein Family: Cullin
• UniProt Gene Name: CUL5
• UniProt Synonym Gene Names: VACM1; CUL-5; VACM-1
• UniProt Entry Name: CUL5_HUMAN

Uniprot Description

CUL5: a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to be involved proteasomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. Member of the cullin family.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: Cul5-RING ubiquitin ligase complex; plasma membrane; cytosol; nucleus

Molecular Function: protein binding; protein heterodimerization activity; ubiquitin protein ligase binding; calcium channel activity; receptor activity

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of cell proliferation; cell proliferation; cytosolic calcium ion homeostasis; viral reproduction; protein ubiquitination; cell cycle arrest; response to osmotic stress; G1/S transition of mitotic cell cycle