Rabbit anti-Human, Mouse CUL4A Polyclonal Antibody | anti-CUL4A antibody

CUL4A, NT (CUL4A, Cullin-4A) (Biotin)

Cat. Num. AAA6290069

• Gene Names: CUL4A; CUL4A
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CUL4A; Polyclonal Antibody; NT (CUL4A; Cullin-4A) (Biotin); Cullin-4A; anti-CUL4A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CUL4A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CUL4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-60 amino acids from the N-terminal region of human CUL4A.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CUL4A Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the CUL4A antibody detected the CUL4A protein (arrow).)

Western Blot (WB)

(CUL4A Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CUL4A antibody detected the CUL4A protein (arrow).)

Western Blot (WB)

(Western blot analysis of CUL4A (arrow) using rabbit polyclonal CUL4A Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CUL4A gene.)

Related Product Information for anti-CUL4A antibody

CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]).

Product Categories/Family for anti-CUL4A antibody

Antibodies; Cullin

References

Aggarwal, P., et al. Cancer Cell 18(4):329-340(2010). Abbas, T., et al. Mol. Cell 40(1):9-21(2010). Lv, X.B., et al. J. Biol. Chem. 285(24):18234-18240(2010). Kerzendorfer, C., et al. Hum. Mol. Genet. 19(7):1324-1334(2010). Melchor, L., et al. Breast Cancer Res. 11 (6), R86 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 57165424
• NCBI GeneID: 8451
• NCBI Accession #: NP_001008895.1; NP_003580.1
• NCBI GenBank Nucleotide #: NM_001008895.1
• UniProt Accession #: Q13619; O75834; Q589T6; Q5TC62; Q6UP08; Q9UP17; A2A2W2
• Molecular Weight: 87,680 Da
• NCBI Official Full Name: cullin-4A isoform 1
• NCBI Official Synonym Full Names: cullin 4A
• NCBI Official Symbol: CUL4A
• NCBI Official Synonym Symbols: CUL4A
• NCBI Protein Information: cullin-4A; CUL-4A; OTTHUMP00000018753; OTTHUMP00000018754
• UniProt Protein Name: Cullin-4A
• Protein Family: Cullin
• UniProt Gene Name: CUL4A
• UniProt Synonym Gene Names: CUL-4A
• UniProt Entry Name: CUL4A_HUMAN

Uniprot Description

CUL4A: Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of p27Kip1/p27kip. Is involved in ubiquitination of HOXA9. Component of multiple DCX (DDB1-CUL4-X-box) E3 ubiquitin- protein ligase complexes that seem to consist of DDB1, CUL4A or CUL4B, RBX1 and a variable substrate recognition component which seems to belong to a protein family described as DCAF (Ddb1- and Cul4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins. Component of the CSA complex (DCX(ERCC8) complex) containing ERCC8, RBX1, DDB1 and CUL4A; the CSA complex interacts with RNA polymerase II; upon UV irradiation it interacts with the COP9 signalosome and preferentially with the hyperphosphorylated form of RNA polymerase II. Component of the DCX(DET1-COP1) complex with the substrate recognition component DET1 and COP1. Component of the DCX(DDB2) complex with the substrate recognition component DDB2. Component of the DCX(DTL) complex with the putative substrate recognition component DTL. Interacts with DDB1, RBX1, RNF7, CTD1, TIP120A/CAND1, SKP2, p27Kip1, MDM2, TP53 and HOXA9. Interacts with DDB2; the interactions with DDB2 and CAND1 are mutually exclusive. Interacts with VPRBP, DTL, DDA1, DCAF6, DCAF4, DCAF16, DCAF17, DET1, WDTC1, DCAF5, DCAF11, WDR24A, RFWD2, PAFAH1B1, ERCC8, GRWD1, FBXW5, RBBP7, GNB2, WSB1, WSB2, NUP43, PWP1, FBXW8, ATG16L1, KATNB1, RBBP4, RBBP5 and DCAF8. May interact with WDR26, WDR51B, SNRNP40, WDR61, WDR76, WDR5. Can self- associate. Interacts with Epstein-Barr virus BPLF1. Belongs to the cullin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 13q34

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; negative regulation of cell proliferation; viral reproduction; somatic stem cell maintenance; positive regulation of cell proliferation; regulation of protein metabolic process; protein ubiquitination; hemopoiesis; cell cycle arrest; DNA repair; negative regulation of granulocyte differentiation; G1/S transition of mitotic cell cycle