Rabbit anti-Human CTSH Polyclonal Antibody | anti-CTSH antibody

CTSH, NT (Cathepsin H, Cathepsin H Mini Chain, Cathepsin H Heavy Chain, Cathepsin H Light Chain, CPSB) (Biotin)

Cat. Num. AAA6467699

• Gene Names: CTSH; CPSB; ACC-4; ACC-5; minichain
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CTSH; Polyclonal Antibody; NT (Cathepsin H; Cathepsin H Mini Chain; Cathepsin H Heavy Chain; Cathepsin H Light Chain; CPSB) (Biotin); anti-CTSH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CTSH.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CTSH antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human CTSH (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CTSH Antibody (N-term) in HepG2 cell line lysates (35ug/lane). CTSH (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CTSH Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CTSH Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CTSH Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CTSH antibody

Antibodies; Cathepsin

References

1. Bunatova, K., Int. J. Biol. Markers 24(1), 47-51 (2009). 2. Decock, J., Int. J. Biol. Markers 23(3), 161-168 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 23110955
• NCBI GeneID: 1512
• NCBI Accession #: NP_004381
• NCBI GenBank Nucleotide #: NM_004390.3
• UniProt Accession #: P09668; Q96NY6; Q9BUM7; B2RBK0
• Molecular Weight: 37,394 Da
• NCBI Official Full Name: pro-cathepsin H preproprotein
• NCBI Official Synonym Full Names: cathepsin H
• NCBI Official Symbol: CTSH
• NCBI Official Synonym Symbols: CPSB; ACC-4; ACC-5; minichain
• NCBI Protein Information: pro-cathepsin H; N-benzoylarginine-beta-naphthylamide hydrolase; aleurain; cathepsin B3; cathepsin BA
• UniProt Protein Name: Pro-cathepsin H
• Protein Family: Pro-cathepsin
• UniProt Gene Name: CTSH
• UniProt Synonym Gene Names: CPSB
• UniProt Entry Name: CATH_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. [provided by RefSeq, Mar 2010]

Uniprot Description

CTSH: Important for the overall degradation of proteins in lysosomes. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.16; Protease

Chromosomal Location of Human Ortholog: 15q25.1

Cellular Component: extracellular space; lysosome; acrosome; outer dense fiber; cytosol

Molecular Function: peptidase activity; protein binding; protein self-association; HLA-A specific activating MHC class I receptor activity; serine-type endopeptidase activity; cysteine-type endopeptidase activity; endopeptidase activity; protein complex binding; caspase activator activity; aminopeptidase activity; kininogen binding; cysteine-type peptidase activity

Biological Process: caspase activation; adaptive immune response; response to retinoic acid; protein destabilization; membrane protein proteolysis; proteolysis; antigen processing and presentation; positive regulation of angiogenesis; positive regulation of cell proliferation; zymogen activation; spermatogenesis; surfactant homeostasis; metanephros development; immune response-regulating signal transduction; positive regulation of cell migration; T cell mediated cytotoxicity; negative regulation of apoptosis