Rabbit CTSA Polyclonal Antibody | anti-CTSA antibody

CTSA (Cathepsin A, Carboxypeptidase C, GSL, GLB2, NGBE, PPCA, PPGB) (FITC)

Cat. Num. AAA6412980

• Gene Names: CTSA; GSL; GLB2; NGBE; PPCA; PPGB
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: CTSA; Polyclonal Antibody; CTSA (Cathepsin A; Carboxypeptidase C; GSL; GLB2; NGBE; PPCA; PPGB) (FITC); anti-CTSA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes CTSA. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-CTSA antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human CTSA.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CTSA antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF-7 cell using CTSA antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-CTSA antibody

Antibodies; Enzymes; Carboxypeptidase

NCBI and Uniprot Product Information

• NCBI GI #: 119395729
• NCBI GeneID: 5476
• NCBI Accession #: NP_000299.2
• NCBI GenBank Nucleotide #: NM_000308.2
• UniProt Accession #: P10619; Q561W6; Q5JZH1; Q96KJ2; Q9BR08; Q9BW68; B2R798
• Molecular Weight: 54,466 Da
• NCBI Official Full Name: lysosomal protective protein isoform a
• NCBI Official Synonym Full Names: cathepsin A
• NCBI Official Symbol: CTSA
• NCBI Official Synonym Symbols: GSL; GLB2; NGBE; PPCA; PPGB
• NCBI Protein Information: lysosomal protective protein; deamidase; urinary kininase; carboxypeptidase C; carboxypeptidase L; carboxypeptidase-L; beta-galactosidase 2; lysosomal carboxypeptidase A; protective protein cathepsin A; carboxypeptidase Y-like kininase; beta-galactosidase
• UniProt Protein Name: Lysosomal protective protein
• Protein Family: Lysosomal protective protein
• UniProt Gene Name: CTSA
• UniProt Synonym Gene Names: PPGB; PPCA
• UniProt Entry Name: PPGB_HUMAN

NCBI Description

This gene encodes a glycoprotein which associates with lysosomal enzymes beta-galactosidase and neuraminidase to form a complex of high molecular weight multimers. The formation of this complex provides a protective role for stability and activity. Deficiencies in this gene are linked to multiple forms of galactosialidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CTSA: Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins. Defects in CTSA are the cause of galactosialidosis (GSL). A lysosomal storage disease associated with a combined deficiency of beta-galactosidase and neuraminidase, secondary to a defect in cathepsin A. All patients have clinical manifestations typical of a lysosomal disorder, such as coarse facies, cherry red spots, vertebral changes, foam cells in the bone marrow, and vacuolated lymphocytes. Three phenotypic subtypes are recognized. The early infantile form is associated with fetal hydrops, edema, ascites, visceromegaly, skeletal dysplasia, and early death. The late infantile type is characterized by hepatosplenomegaly, growth retardation, cardiac involvement, and a normal or mildly affected mental state. The juvenile/adult form is characterized by myoclonus, ataxia, angiokeratoma, mental retardation, neurologic deterioration, absence of visceromegaly, and long survival. Belongs to the peptidase S10 family.

Protein type: Endoplasmic reticulum; Mitochondrial; Protease; EC 3.4.16.5

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: nucleoplasm; lysosomal lumen; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; lysosome

Molecular Function: serine carboxypeptidase activity; carboxypeptidase activity; enzyme activator activity

Biological Process: positive regulation of catalytic activity; intracellular protein transport; cellular protein metabolic process; sphingolipid metabolic process; dolichol-linked oligosaccharide biosynthetic process; protein amino acid N-linked glycosylation via asparagine; glycosphingolipid metabolic process; post-translational protein modification; proteolysis

Disease: Galactosialidosis