Rabbit anti-Human, Mouse CTR9 Polyclonal Antibody | anti-CTR9 antibody

CTR9 Antibody

Cat. Num. AAA9630043

• Gene Names: CTR9; TSBP; p150; SH2BP1; p150TSP
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CTR9; Polyclonal Antibody; CTR9 Antibody; Ctr 9; Ctr9 Paf1/RNA polymerase II complex component; homolog; Paf1/RNA polymerase II complex componen; S. cerevisiae; homolog of; CTR9_HUMAN; KIAA0155; p150; p150(TSP); p150TSP.; Paf1/RNA polymerase II complex component homolog Ctr9; RNA polymerase associated protein CTR9 homolog; RNA polymerase-associated protein CTR9 homolog; SH2 binding phosphoprotein; SH2 domain binding protein 1 (tetratricopeptide repeat containing); SH2 domain binding protein 1; SH2 domain-binding protein 1; SH2-binding phosphoprotein of 150 kDa; SH2BP1; Tetratricopeptide repeat-containing; Tetratricopeptide-containing SH2-binding phosphoprotein 150-KD; TPR containing; TSBP; anti-CTR9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CTR9 Antibody detects endogenous levels of CTR9.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CTR9 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human CTR9.
• Conjugation: Unconjugated
• Subunit Structure: Component of the PAF1 complex, which consists of CDC73, PAF1, LEO1, CTR9, RTF1 and WDR61. The PAF1 complex interacts with PHF5A (By similarity). Interacts with KMT2A/MLL1. Interacts with STAT3 (By similarity). Interacts with SETD5 (By similarity). Interacts with ERCC6.
• Tissue Specificity: Widely expressed.
• Function: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3) and dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of the SET1 complex. Involved in transcriptional regulation of IL6-responsive genes and in JAK-STAT pathway; may regulate DNA-association of STAT3 (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-CTR9 antibody

Nucleus speckle; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 74758318
• NCBI GeneID: 9646
• UniProt Accession #: Q6PD62; Q15015; D3DQV8
• Molecular Weight: 1173
• NCBI Official Full Name: RNA polymerase-associated protein CTR9 homolog
• NCBI Official Synonym Full Names: CTR9, Paf1/RNA polymerase II complex component
• NCBI Official Symbol: CTR9
• NCBI Official Synonym Symbols: TSBP; p150; SH2BP1; p150TSP
• NCBI Protein Information: RNA polymerase-associated protein CTR9 homolog; SH2 domain-binding protein 1; TPR-containing, SH2-binding phosphoprotein; Ctr9, Paf1/RNA polymerase II complex component, homolog; SH2 domain binding protein 1 (tetratricopeptide repeat containing)
• UniProt Protein Name: RNA polymerase-associated protein CTR9 homolog
• Protein Family: RNA polymerase-associated protein
• UniProt Gene Name: CTR9
• UniProt Synonym Gene Names: KIAA0155; SH2BP1
• UniProt Entry Name: CTR9_HUMAN

NCBI Description

CTR9, parafibromin (CDC73; MIM 607393), LEO1 (MIM 610507), and PAF1 (MIM 610506) form the PAF protein complex, which associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).[supplied by OMIM, Mar 2008]

Uniprot Description

SH2BP1: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3) and dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of the SET1 complex. Involved in transcriptional regulation of IL6-responsive genes and in JAK-STAT pathway; may regulate DNA-association of STAT3.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 11p15.3

Cellular Component: nuclear speck

Molecular Function: protein binding; SH2 domain binding

Biological Process: histone monoubiquitination; negative regulation of myeloid cell differentiation; Wnt receptor signaling pathway; transcription, DNA-dependent; endodermal cell fate commitment; positive regulation of RNA elongation from RNA polymerase II promoter; histone H2B ubiquitination; stem cell maintenance; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; JAK-STAT cascade