Rabbit anti-Human CtIP Polyclonal Antibody | anti-CtIP antibody

CtIP Antibody

Cat. Num. AAA9613568

• Gene Names: RBBP8; RIM; COM1; CTIP; JWDS; SAE2; SCKL2
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: CtIP; Polyclonal Antibody; CtIP Antibody; COM1; COM1_HUMAN; CtBP interacting protein; CtBP-interacting protein; DNA endonuclease RBBP8; JWDS; RB binding protein 8 endonuclease; RBBP-8; RBBP8; Retinoblastoma-binding protein 8; Retinoblastoma-interacting protein and myosin-like; Rim; SAE2; SCKL2; Sporulation in the absence of SPO11 protein 2 homolog; anti-CtIP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CtIP Antibody detects endogenous levels of total CtIP.; Tissue Specificity: Expressed in ER-positive breast cancer lines, but tends to be down-regulated ER-negative cells (at protein level).
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CtIP antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human CtIP.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Acetylated. Deacetylation by SIRT6 upon DNA damage promotes DNA end resection.Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylation at Thr-847 by CDK1 is essential for the recruitment to DNA and the DNA repair function. Phosphorylated on Ser-327 as cells enter G2 phase. This phosphorylation is required for binding BRCA1 and for the G2/M DNA damage transition checkpoint control. Phosphorylation at Thr-315, probably catalyzed by CDK2, is required for PIN1-binding, while phosphorylation at Ser-276 serves as a PIN1 isomerization site. Phosphorylation at Thr-315 is cell-cycle dependent. It steadily increases during S phase, peaks at late S/G2 phase, and drops at G1.Ubiquitinated. Ubiquitination at multiple sites by BRCA1 (via its N-terminal RING domain) does not lead to its proteasomal degradation but instead the ubiquitinated RBBP8 binds to chromatin following DNA damage and may play a role in G2/M checkpoint control. Ubiquitinated by RNF138 at its N-terminus. Ubiquitinated through 'Lys-48' by the E3 CUL3-KLHL15 complex; this modification leads to proteasomal degradation. Ubiquitinated by the E3 FZR1/APC/C complex; this modification leads to proteasomal degradation.
• Subunit Structure: Homodimer; dimerizes via the coiled coil domain. Interacts (via the PXDLS motif) with CTBP1; the interaction is disrupted via binding of the adenovirus E1A to CTBP1. Component of the BRCA1-RBBP8 complex. Interacts (the Ser-327 phosphorylated form) with BRCA1 (via the C-terminal BRCA1 domains): the interaction occurs in the G2 phase, ubiquitinates RBBP8 and involves RBBP8 in BRCA1-dependent G2/M checkpoint control on DNA damage. Interacts with RB1. Interacts with the MRN complex. Interacts directly with MRE11; the interaction is required for efficient homologous recombination (HR) and regulation of the MRN complex. Interacts directly with RAD50. Interacts directly with NBN. Interacts with SIRT6; the interaction deacetylates RBBP8 upon DNA damage. Interacts with LM04 (via the LIM zinc-binding 1 domain). Interacts with SIAH1. Interacts with RNF138. Interacts with EXD2. Interacts with CUL3 and KLHL15; this interaction leads to RBBP8 proteasomal degradation. Directly interacts with PIN1; this interaction depends upon RBBP8 phosphorylation, predominantly at Thr-315. Interacts with FZR1; this interaction leads to APC/C-mediated RBBP8 proteasomal degradation. Interacts with AUNIP; leading to recruit RBBP8 to sites of DNA damage. Interacts with SAMHD1.
• Similarity: The PXDLS motif binds to a cleft in CtBP proteins.The damage-recruitment motif is required for DNA binding and translocation to sites of DNA damage.Belongs to the COM1/SAE2/CtIP family.
• Subcellular Location: Nucleus. Chromosome.; Note: Associates with sites of DNA damage in S/G2 phase (PubMed:10764811, PubMed:25349192). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Related Product Information for anti-CtIP antibody

Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 4506441
• NCBI GeneID: 5932
• NCBI Accession #: NP_002885.1
• NCBI GenBank Nucleotide #: NM_002894.2
• UniProt Accession #: Q99708; O75371; Q8NHQ3; A6NKN2; A8K8W6; E7ETY1
• Molecular Weight: 101,942 Da
• NCBI Official Full Name: DNA endonuclease RBBP8 isoform a
• NCBI Official Synonym Full Names: retinoblastoma binding protein 8
• NCBI Official Symbol: RBBP8
• NCBI Official Synonym Symbols: RIM; COM1; CTIP; JWDS; SAE2; SCKL2
• NCBI Protein Information: DNA endonuclease RBBP8; RBBP-8; CTBP-interacting protein; sporulation in the absence of SPO11 protein 2 homolog
• UniProt Protein Name: DNA endonuclease RBBP8
• UniProt Gene Name: RBBP8
• UniProt Synonym Gene Names: CTIP; CtIP; RBBP-8; RIM; SAE2
• UniProt Entry Name: COM1_HUMAN

NCBI Description

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CtIP: a transcription factor containing C2H2 zinc fingers. May modulate the functions ascribed to BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. Interacts with CTBP, with the C-terminal (BRCT) domains of BRCA1, and with the retinoblastoma protein. Abundantly expressed in brain and the immune system and associated with immune system malignancies. Ionizing radiation induces its hyperphosphorylation and dissociation from BRCA1 in an ATM-dependent manner.

Protein type: Transcription, coactivator/corepressor; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: nucleoplasm; transcriptional repressor complex; nucleolus; nucleus

Molecular Function: protein binding; single-stranded DNA specific endodeoxyribonuclease activity; damaged DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; mitosis; meiotic cell cycle; cell division; blastocyst hatching; negative regulation of transcription from RNA polymerase II promoter; DNA repair; G2/M transition DNA damage checkpoint; cell cycle checkpoint; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination; G1/S transition of mitotic cell cycle

Disease: Jawad Syndrome; Seckel Syndrome 2