Rabbit anti-Human CTGF Polyclonal Antibody | anti-CTGF antibody

CTGF, CT (Connective Tissue Growth Factor, Hypertrophic Chondrocyte-specific Protein 24, Insulin-like Growth Factor-binding Protein 8, IGF-binding Protein 8, IGFBP-8, IBP-8, CCN Family Member 2, CCN2, HCS24, IGFBP8) (AP)

Cat. Num. AAA6470887

• Gene Names: CTGF; CCN2; NOV2; HCS24; IGFBP8
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation
• Synonyms: CTGF; Polyclonal Antibody; CT (Connective Tissue Growth Factor; Hypertrophic Chondrocyte-specific Protein 24; Insulin-like Growth Factor-binding Protein 8; IGF-binding Protein 8; IGFBP-8; IBP-8; CCN Family Member 2; CCN2; HCS24; IGFBP8) (AP); anti-CTGF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CTGF.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-CTGF antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: IHC-P: 1:10-1:50; WB: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 315-349 in the C-terminal region of human CTGF, UniProt Accession #P29279. Species sequence homology: mouse, rat, bovine and porcine
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS627860 in NCI-H460 cell line lysates (35ug/lane). CTGF (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted withMBS627860, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Product Categories/Family for anti-CTGF antibody

Antibodies; Growth Factors; Cytokines

References

1. Li, M.H., Mol. Cancer Res. 6(10), 1649-1656 (2008). 2. Bogatkevich, G.S., Am. J. Physiol. Lung Cell Mol. Physiol. 295(4), L603-L611 (2008). 3. Zhang, P., Tohoku J. Exp. Med. 215(3), 199-206 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4503123
• NCBI GeneID: 1490
• NCBI Accession #: NP_001892
• NCBI GenBank Nucleotide #: NM_001901.2
• UniProt Accession #: P29279; Q6LCY0; Q96A79; Q96QX2; Q9UDL6; E1P578
• Molecular Weight: 37.7kDa (344aa)
• NCBI Official Full Name: connective tissue growth factor
• NCBI Official Synonym Full Names: connective tissue growth factor
• NCBI Official Symbol: CTGF
• NCBI Official Synonym Symbols: CCN2; NOV2; HCS24; IGFBP8
• NCBI Protein Information: connective tissue growth factor
• UniProt Protein Name: Connective tissue growth factor
• Protein Family: Connective tissue growth factor
• UniProt Gene Name: CTGF
• UniProt Synonym Gene Names: CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8
• UniProt Entry Name: CTGF_HUMAN

NCBI Description

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]

Uniprot Description

CTGF: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cell adhesion

Chromosomal Location of Human Ortholog: 6q23.1

Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; cis-Golgi network; intracellular membrane-bound organelle; perinuclear region of cytoplasm; plasma membrane; extracellular region; cell cortex; cytosol

Molecular Function: integrin binding; heparin binding; insulin-like growth factor binding; protein binding; growth factor activity; fibronectin binding

Biological Process: response to peptide hormone stimulus; epidermis development; response to anoxia; cell-matrix adhesion; positive regulation of collagen biosynthetic process; positive regulation of caspase activity; positive regulation of JNK cascade; cellular lipid metabolic process; response to estradiol stimulus; cell-cell signaling; positive regulation of stress fiber formation; positive regulation of cell proliferation; response to glucose stimulus; tissue homeostasis; response to wounding; angiogenesis; regulation of cell growth; cell differentiation; cell adhesion; positive regulation of cell activation; cartilage condensation; integrin-mediated signaling pathway; transcription initiation from RNA polymerase II promoter; cell migration; ossification; fibroblast growth factor receptor signaling pathway; organ senescence; response to mineralocorticoid stimulus; response to amino acid stimulus; gene expression; regulation of chondrocyte differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of cell differentiation; lung development