Goat CTDSP1 Polyclonal Antibody | anti-CTDSP1 antibody

Goat anti-CTDSP1 Antibody

Cat. Num. AAA420988

• Gene Names: CTDSP1; NIF3; SCP1; NLIIF; NLI-IF
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CTDSP1; Polyclonal Antibody; Goat anti-CTDSP1 Antibody; CTD (carboxy-terminal domain; RNA polymerase II; polypeptide A) small phosphatase 1; NLIIF; SCP1; nuclear LIM interactor-interacting factor; small CTD phosphatase 1; CTDSP1 antibody; polypeptide A) small phosphatase 1 antibody; NLIIF antibody; SCP1 antibody; nuclear LIM interactor-interacting factor antibody; small CTD phosphatase 1 antibody; anti-CTDSP1 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: RGKGDQKSAASQK
• Sequence Length: 261

• Applicable Applications for anti-CTDSP1 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Preliminary experiments gave an approx 60kDa band in human spleen, lung and placenta lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 29.2kDa according to NP_067021.1 and 29.1kDa according to NP_872580.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-RGKGDQKSAASQK, from the internal region of the protein sequence according to NP_067021.1; NP_872580.1.
• Epitope: Internal region
• Note: This antibody is expected to recognise both reported isoforms (NP_067021.1and NP_872580.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 10864009
• NCBI GeneID: 58190
• NCBI Accession #: NP_067021.1; NP_872580.1
• NCBI GenBank Nucleotide #: NM_021198.2
• Molecular Weight: 29,128 Da
• NCBI Official Full Name: carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 isoform 1
• NCBI Official Synonym Full Names: CTD small phosphatase 1
• NCBI Official Symbol: CTDSP1
• NCBI Official Synonym Symbols: NIF3; SCP1; NLIIF; NLI-IF
• NCBI Protein Information: carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
• UniProt Protein Name: Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
• Protein Family: Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase
• UniProt Gene Name: CTDSP1
• UniProt Synonym Gene Names: NIF3; NLIIF; SCP1; NLI-IF; NLI-interacting factor 3; SCP1; Small CTD phosphatase 1
• UniProt Entry Name: CTDS1_HUMAN

NCBI Description

This gene encodes a member of the small C-terminal domain phosphatase (SCP) family of nuclear phosphatases. These proteins play a role in transcriptional regulation through specific dephosphorylation of phosphoserine 5 within tandem heptapeptide repeats of the C-terminal domain of RNA polymerase II. The encoded protein plays a role in neuronal gene silencing in non-neuronal cells, and may also inhibit osteoblast differentiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

CTDSP1: Preferentially catalyzes the dephosphorylation of 'Ser- 5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleoplasm; nucleus

Molecular Function: CTD phosphatase activity; metal ion binding; protein binding

Biological Process: negative regulation of neuron differentiation; negative regulation of protein amino acid phosphorylation; protein amino acid dephosphorylation; regulation of transcription from RNA polymerase II promoter