Rabbit anti-Human CTDP1 Polyclonal Antibody | anti-CTDP1 antibody

CTDP1, NT (RNA Polymerase II Subunit A C-terminal Domain Phosphatase, TFIIF-associating CTD Phosphatase, FCP1)

Cat. Num. AAA631073

• Gene Names: CTDP1; FCP1; CCFDN
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CTDP1; Polyclonal Antibody; NT (RNA Polymerase II Subunit A C-terminal Domain Phosphatase; TFIIF-associating CTD Phosphatase; FCP1); Anti -CTDP1; anti-CTDP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CTDP1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-CTDP1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human CTDP1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CTDP1 Antibody (N-term) in 293 cell line lysates (35ug/lane). CTDP1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue with CTDP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of WiDr cells using CTDP1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CTDP1 antibody

CTDP1 is a protein which interacts with the carboxy-terminus of transcription initiation factor TFIIF, a transcription factor which regulates elongation as well as initiation by RNA polymerase II. The protein may also represent a component of an RNA polymerase II holoenzyme complex.

Product Categories/Family for anti-CTDP1 antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 30962890
• NCBI GeneID: 9150
• UniProt Accession #: Q9Y5B0; Q7Z644; Q96BZ1; Q9Y6F5; A8MY97
• Molecular Weight: 104,399 Da
• NCBI Official Full Name: CTDP1 protein
• NCBI Official Synonym Full Names: CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1
• NCBI Official Symbol: CTDP1
• NCBI Official Synonym Symbols: FCP1; CCFDN
• NCBI Protein Information: RNA polymerase II subunit A C-terminal domain phosphatase; OTTHUMP00000163841; OTTHUMP00000163842; serine phosphatase FCP1a; TFIIF-associating CTD phosphatase 1; CTD of POLR2A, phosphatase of, subunit 1; transcription factor IIF-associating CTD phosphatase 1
• UniProt Protein Name: RNA polymerase II subunit A C-terminal domain phosphatase
• UniProt Gene Name: CTDP1
• UniProt Synonym Gene Names: FCP1
• UniProt Entry Name: CTDP1_HUMAN

NCBI Description

This gene encodes a protein which interacts with the carboxy-terminus of the RAP74 subunit of transcription initiation factor TFIIF, and functions as a phosphatase that processively dephosphorylates the C-terminus of POLR2A (a subunit of RNA polymerase II), making it available for initiation of gene expression. Mutations in this gene are associated with congenital cataracts, facial dysmorphism and neuropathy syndrome (CCFDN). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Uniprot Description

CTDP1: a phospho-serine/threonine protein phosphatase which interacts with the carboxy-terminus of transcription initiation factor TFIIF-alpha, a transcription factor which regulates elongation as well as initiation by RNA polymerase II. Its phosphorylation increases binding to TFIIF-alpha, affecting transcription elongation. Alternative splicing results in two different isoforms.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor); Transcription initiation complex

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: nucleoplasm; spindle pole; centrosome; cytoplasm; spindle; spindle midzone; midbody; actin cytoskeleton

Molecular Function: DNA-directed RNA polymerase activity; CTD phosphatase activity

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; cell division; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression; protein amino acid dephosphorylation; exit from mitosis

Disease: Congenital Cataracts, Facial Dysmorphism, And Neuropathy