Western Blot (WB)
((0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat RPS19 Polyclonal Antibody | anti-RPS19 antibody
Goat anti-RPS19 Antibody
Western Blot: Approx 15kDa band observed in lysates of cell lines Daudi and K562 (calculated MW of 16.1kDa according to NP_001013.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19E family of ribosomal proteins. It is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia (DBA), a constitutional erythroblastopenia characterized by absent or decreased erythroid precursors, in a subset of patients. This suggests a possible extra-ribosomal function for this gene in erythropoietic differentiation and proliferation, in addition to its ribosomal function. Higher expression levels of this gene in some primary colon carcinomas compared to matched normal colon tissues has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Uniprot Description
RPS19: Required for pre-rRNA processing and maturation of 40S ribosomal subunits. Defects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1). DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein S19e family.
Protein type: Translation; Ribosomal
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; nucleolus; nucleoplasm; ribosome
Molecular Function: fibroblast growth factor binding; protein binding; protein homodimerization activity; protein kinase binding; structural constituent of ribosome
Biological Process: erythrocyte differentiation; maturation of SSU-rRNA; maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); monocyte chemotaxis; mRNA catabolic process, nonsense-mediated decay; Notch signaling pathway; nucleolus organization and biogenesis; positive regulation of cell motility; protein tetramerization; response to extracellular stimulus; ribosomal small subunit assembly and maintenance; ribosomal small subunit biogenesis and assembly; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription
Disease: Diamond-blackfan Anemia 1
Research Articles on RPS19
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Product Notes
The RPS19 rps19 (Catalog #AAA420809) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RPS19 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's RPS19 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 15kDa band observed in lysates of cell lines Daudi and K562 (calculated MW of 16.1kDa according to NP_001013.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the RPS19 rps19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KMVEKDQDGG RK. It is sometimes possible for the material contained within the vial of "RPS19, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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