Rabbit anti-Human, Mouse CTBP1 Polyclonal Antibody | anti-CTBP1 antibody

CTBP1, CT (CTBP1, CTBP, C-terminal-binding protein 1) (FITC)

Cat. Num. AAA6289663

• Gene Names: CTBP1; BARS
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CTBP1; Polyclonal Antibody; CT (CTBP1; CTBP; C-terminal-binding protein 1) (FITC); C-terminal-binding protein 1; anti-CTBP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CTBP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CTBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 412-442 amino acids from the C-terminal region of human CTBP1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CTBP1 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the CTBP1 antibody detected the CTBP1 protein (arrow).)

Related Product Information for anti-CTBP1 antibody

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.

Product Categories/Family for anti-CTBP1 antibody

Antibodies; Binding Proteins

References

Roukens, M.G., et al. Nat. Cell Biol. 12(10):933-942(2010). Choi, H.J., et al. Biochem. Biophys. Res. Commun. 400(3):396-402(2010). Merrill, J.C., et al. J. Mol. Biol. 398(5):657-671(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4557497
• NCBI GeneID: 1487
• NCBI Accession #: NP_001319.1
• NCBI GenBank Nucleotide #: NM_001328.2
• UniProt Accession #: Q13363; Q4W5N3; Q7Z2Q5
• Molecular Weight: 46,405 Da
• NCBI Official Full Name: C-terminal-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: C-terminal binding protein 1
• NCBI Official Symbol: CTBP1
• NCBI Official Synonym Symbols: BARS
• NCBI Protein Information: C-terminal-binding protein 1; brefeldin A-ribosylated substrate
• UniProt Protein Name: C-terminal-binding protein 1
• UniProt Gene Name: CTBP1
• UniProt Synonym Gene Names: CTBP; CtBP1
• UniProt Entry Name: CTBP1_HUMAN

NCBI Description

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

CTBP1: Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity. Homo- or heterodimer. Heterodimer with CTBP2. Interacts with PRDM16; the interaction represses white adipose tissue (WAT)- specific genes expression. Interacts with GLIS2, FOXP2, HDAC4, HDAC5, HDAC9 and ZNF217. Interacts with adenovirus E1A protein (via its C-terminus); the interaction disrupts the interaction of CTBP1 with RBBP8. Interacts with Epstein-Barr virus EBNA3 and EBNA6. Interacts with ELK3 (via its PXDLS motif). Interacts with RBBP8 (via its PXDLS motif); the interaction is disrupted by binding to adenovirus E1A. Interacts with FOXP1, HIPK2, PNN, NRIP1, MECOM, ZNF366, ZFHX1B and WIZ. Interaction with SATB1 (non-acetylated form); the interaction stabilizes its attachment to DNA and promotes transcription repression. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.-; Oxidoreductase; Cell cycle regulation; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; cytosol; nucleus

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; protein homodimerization activity; transcription factor activity; NAD binding; transcription factor binding; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process: negative regulation of cell proliferation; positive regulation of histone deacetylation; transcription, DNA-dependent; regulation of cell cycle; white fat cell differentiation; negative regulation of transcription from RNA polymerase II promoter; viral genome replication; negative regulation of transcription, DNA-dependent; Golgi organization and biogenesis; protein amino acid phosphorylation; negative regulation of histone acetylation

Disease: Wolf-hirschhorn Syndrome