Rabbit anti-Human CSNK2A2 Polyclonal Antibody | anti-CSNK2A2 antibody

Anti-CSNK2A2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8104575

• Gene Names: CSNK2A2; CK2A2; CSNK2A1; CK2alpha'
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: CSNK2A2; Polyclonal Antibody; Anti-CSNK2A2 Antibody; Rabbit Polyclonal; CSNK2A2 Antibody; Rabbit PAb; Antigen Affinity Purified; casein kinase 2; alpha prime polypeptide; Anti-CK2A2 Antibody; Anti-CSNK2A1 Antibody; anti-CSNK2A2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CSNK2A2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CSNK2A2 antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; ICC/IF: 1:300-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human CSNK2A2 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CSNK2A2 (rh CSNK2A2). CSNK2A2 specific IgG was purified by Human CSNK2A2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CSNK2A2 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:41 kDaObserved band size:45 kDa(We are unsure as to the identity of these extra bands.))

Immunofluorescence (IF)

(Immunofluorescence staining of CSNK2A2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CSNK2A2 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)

Immunoprecipitation (IP)

(CSNK2A2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate4 uL anti-CSNK2A2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CSNK2A2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size: 41 kDa)

Related Product Information for anti-CSNK2A2 antibody

Casein kinase II subunit alpha', also known as CSNK2A2 and CK2A2, is a member of the protein kinase superfamily, Ser/Thr protein kinase family and CK2 subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. CSNK2A2 is a tetramer composed of an alpha chain, an alpha' and two beta chains. It is also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Protein kinase casein kinase II (Ck2) is a cyclic-AMP and calcium-independent serine-threonine kinase that is composed of two catalytic subunits (alpha and alpha') and two regulatory beta-subunits. Ck2 is not a casein kinase in vivo, but over 1 substrates are known. The highly conserved amino acid sequences of its subunits and their broad expression suggest that Ck2 may have a fundamental role in cell function. Ck2 has been implicated in DNA replication, regulation of basal and inducible transcription, translation and control of metabolism. The Ck2alpha and Ck2alpha' isoforms (products of the genes Csnk2a1 and Csnk2a2, respectively) are highly homologous, the reason for their redundancy and evolutionary conservation is unknown. CSNK2A2 may be a candidate gene for these inherited syndromes.

References

Xu X. et al., 1999, Nat Genet. 23 (1):118-21. Aasland M. et al., 2000, Anim Genet. 31 (2):131-4. Escalier D. et al., 2003, Mol Reprod Dev. 66 (2):190-201. Ackermann K. et al., 2005, Mol Cell Biochem. 274 (1-2):91-101.

NCBI and Uniprot Product Information

• NCBI GI #: 4503097
• NCBI GeneID: 1459
• NCBI Accession #: NP_001887.1
• NCBI GenBank Nucleotide #: NM_001896.2
• UniProt Accession #: P19784
• Molecular Weight: 41kDa
• NCBI Official Full Name: casein kinase II subunit alpha'
• NCBI Official Synonym Full Names: casein kinase 2 alpha 2
• NCBI Official Symbol: CSNK2A2
• NCBI Official Synonym Symbols: CK2A2; CSNK2A1; CK2alpha'
• NCBI Protein Information: casein kinase II subunit alpha'
• UniProt Protein Name: Casein kinase II subunit alpha'
• Protein Family: Casein kinase
• UniProt Gene Name: CSNK2A2
• UniProt Synonym Gene Names: CK2A2; CK II alpha'
• UniProt Entry Name: CSK22_HUMAN

Uniprot Description

CK2A2: Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; CK2 family

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: PcG protein complex; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; protein N-terminus binding; ATP binding

Biological Process: axon guidance; Wnt receptor signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis; mitotic cell cycle; protein amino acid phosphorylation