Rabbit CSN8 Polyclonal Antibody | anti-COPS8 antibody

CSN8 Antibody

Cat. Num. AAA150159

• Gene Names: COPS8; COP9; CSN8; SGN8
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: CSN8 Antibody is affinity chromatography purified via peptide column.
• Synonyms: CSN8; Polyclonal Antibody; CSN8 Antibody; COP9; SGN8; COP9 signalosome complex subunit 8; COP9 homolog; COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis); anti-COPS8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: CSN8 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 209

• Applicable Applications for anti-COPS8 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: CSN8 antibody can be used for detection of CSN8 by Western blot at 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CSN8 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human CSN8.
• Buffer: CSN8 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CSN8 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CSN8 in Human liver lysate with CSN8 antibody at 2 μg/mL.)

Related Product Information for anti-COPS8 antibody

CSN8 Antibody: The COP9 signalosome (CSN) is an evolutionarily conserved protein complex of the eight subunits that interacts with deubiquitinating enzymes and protein kinases and is highly homologous to the lid sub-complex of 26S proteasome. The CSN complex is an essential regulator of the ubiquitin conjugation pathway by mediating the deneddylation of the SCF-type E3 ligase complexes, which leads to a decrease in ubiquitin ligase activity of SCF-comlpexes such as SCF, CSA or DDB2. It is also involved in phosphorylation of p53, c-jun/JUN, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN8 encodes the smallest and the least conserved but first identified subunit of CSN. Recent studies show CSN8 is essential for Drosophila development and is essential for peripheral T cell homeostasis and antigen receptor-induced entry into the cell cycle from quiescence.

NCBI and Uniprot Product Information

• NCBI GI #: 5729779
• NCBI GeneID: 10920
• NCBI Accession #: NP_006701
• NCBI GenBank Nucleotide #: NM_006710.4
• UniProt Accession #: Q99627; Q53QS9; A8K1H6
• Molecular Weight: 17,909 Da
• NCBI Official Full Name: COP9 signalosome complex subunit 8 isoform 1
• NCBI Official Synonym Full Names: COP9 signalosome subunit 8
• NCBI Official Symbol: COPS8
• NCBI Official Synonym Symbols: COP9; CSN8; SGN8
• NCBI Protein Information: COP9 signalosome complex subunit 8; hCOP9; COP9 homolog; signalosome subunit 8; JAB1-containing signalosome subunit 8; COP9 constitutive photomorphogenic homolog subunit 8
• UniProt Protein Name: COP9 signalosome complex subunit 8
• UniProt Gene Name: COPS8
• UniProt Synonym Gene Names: CSN8; SGN8; Signalosome subunit 8; hCOP9
• UniProt Entry Name: CSN8_HUMAN

NCBI Description

The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

COPS8: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Belongs to the CSN8 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: nucleoplasm; signalosome; perinuclear region of cytoplasm; cytoplasm; nucleus

Biological Process: negative regulation of cell proliferation; cullin deneddylation; activation of NF-kappaB-inducing kinase