Western Blot (WB)
(Western blot analysis of CSF2RB using Jurkat whole lysates.)
Rabbit anti-Human, Mouse CSF2RB Polyclonal Antibody | anti-CSF2RB antibody
CSF2RB Antibody
Gene Names
CSF2RB; CD131; IL3RB; IL5RB; SMDP5; CDw131; betaGMR
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Peptide affinity purification.
Synonyms
CSF2RB; Polyclonal Antibody; CSF2RB Antibody; granulocyte-macrophage colony-stimulating factor receptor; beta; Beta-C; CD131; CDw131; Colony stimulating factor 2 receptor beta; Colony stimulating factor 2 receptor beta low affinity (granulocyte macrophage); Common beta chain; Csf2rb; Cytokine receptor common beta chain; Cytokine receptor common beta chain precursor (CDw131 antigen); Cytokine receptor common subunit beta; GM CSF/IL 3/IL 5 receptor common beta chain; GM-CSF/IL-3/IL-5 receptor common beta subunit; IL3RB; IL3RB_HUMAN; IL5RB; Interleukin 3 receptor; Interleukin 3 receptor/granulocyte macrophage colony stimulating factor 3 receptor beta (high affinity); Interleukin 5 receptor; SMDP5; stimulating factor 3 receptor; beta (high affinity); anti-CSF2RB antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CSF2RB antibody detects endogenous levels of total CSF2RB
Purity/Purification
Peptide affinity purification.
Form/Format
Liquid
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
897
Applicable Applications for anti-CSF2RB antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IF/ICC: 1:100-1:500
ELISA (peptide): 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
IF/ICC: 1:100-1:500
ELISA (peptide): 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Immunogen
A synthesized peptide derived from human CSF2RB, corresponding to a region within C-terminal amino acids.
Important
For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of CSF2RB using Jurkat whole lysates.)
Western Blot (WB)
(Western blot analysis of CSF2RB using Jurkat whole lysates.)
Immunofluorescene (IF)
(MBS9608262 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9608262 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-CSF2RB antibody
High affinity recptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
130 kDa
NCBI Official Full Name
cytokine receptor common subunit beta
NCBI Official Synonym Full Names
colony stimulating factor 2 receptor beta common subunit
NCBI Official Symbol
CSF2RB
NCBI Official Synonym Symbols
CD131; IL3RB; IL5RB; SMDP5; CDw131; betaGMR
NCBI Protein Information
cytokine receptor common subunit beta
UniProt Protein Name
Cytokine receptor common subunit beta
Protein Family
UniProt Gene Name
CSF2RB
UniProt Synonym Gene Names
IL3RB; IL5RB
NCBI Description
The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP). [provided by RefSeq, Jul 2008]
Uniprot Description
High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.
Research Articles on CSF2RB
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Product Notes
The CSF2RB csf2rb (Catalog #AAA9608262) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CSF2RB Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CSF2RB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000-3000 IF/ICC: 1:100-1:500 ELISA (peptide): 1:20000-1:40000 *The optimal dilutions should be determined by the end user. Researchers should empirically determine the suitability of the CSF2RB csf2rb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CSF2RB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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