Rabbit anti-Human THOC4 Polyclonal Antibody | anti-THOC4 antibody

THOC4, ID (ALYREF, ALY, BEF, THOC4, THO complex subunit 4, Ally of AML-1 and LEF-1, Aly/REF export factor, Transcriptional coactivator Aly/REF, bZIP-enhancing factor BEF) (PE)

Cat. Num. AAA6355458

• Gene Names: ALYREF; ALY; BEF; REF; THOC4; ALY/REF
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: THOC4; Polyclonal Antibody; ID (ALYREF; ALY; BEF; THO complex subunit 4; Ally of AML-1 and LEF-1; Aly/REF export factor; Transcriptional coactivator Aly/REF; bZIP-enhancing factor BEF) (PE); ALYREF; bZIP-enhancing factor BEF; anti-THOC4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-THOC4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: THOC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-89 amino acids from the Central region of human THOC4.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(THOC4 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the THOC4 antibody detected the THOC4 protein (arrow).)

Related Product Information for anti-THOC4 antibody

THOC4 is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.

Product Categories/Family for anti-THOC4 antibody

Antibodies; Transcription Factors

References

Corbin-Lickfett, K.A., et al. J. Virol. 84(5):2212-2222(2010). Souki, S.K., et al. J. Virol. 83(17):8970-8975(2009). Johnson, L.A., et al. J. Virol. 83(13):6335-6346(2009). Colgan, K.J., et al. J. Gen. Virol. 90 (PT 6), 1455-1460 (2009): Katahira, J., et al. EMBO J. 28(5):556-567(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 238776833
• NCBI GeneID: 10189
• NCBI Accession #: NP_005773.3
• NCBI GenBank Nucleotide #: NM_005782.3
• UniProt Accession #: Q86V81; O43672
• Molecular Weight: 26,888 Da
• NCBI Official Full Name: THO complex subunit 4
• NCBI Official Synonym Full Names: Aly/REF export factor
• NCBI Official Symbol: ALYREF
• NCBI Official Synonym Symbols: ALY; BEF; REF; THOC4; ALY/REF
• NCBI Protein Information: THO complex subunit 4; tho4; THO complex 4; bZIP enhancing factor; ally of AML-1 and LEF-1; bZIP-enhancing factor BEF; transcriptional coactivator Aly/REF
• UniProt Protein Name: THO complex subunit 4
• UniProt Gene Name: ALYREF
• UniProt Synonym Gene Names: ALY; BEF; THOC4; Tho4
• UniProt Entry Name: THOC4_HUMAN

NCBI Description

The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

THOC4: a chaperone protein that promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains thus promoting transcriptional activation. Plays a role in mRNA processing and export. May function as scaffold that mediates interactions between proteins and/or RNA. Integral part of the THO/TREX complex that is recruited to transcribed genes and travels with the RNA polymerase during elongation. Is part of the exon junction complex that remains associated with spliced mRNA and plays an important role in mRNA export and nonsense-mediated RNA decay. Nuclear localization, translocates to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Two differentially spliced isoforms have been described.

Protein type: Spliceosome; Transcription, coactivator/corepressor; RNA-binding; Chaperone

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; membrane; intracellular membrane-bound organelle; nuclear speck; cytosol; nucleus

Molecular Function: protein binding; nucleotide binding; single-stranded DNA binding

Biological Process: osteoblast differentiation; nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; mRNA export from nucleus; positive regulation of RNA elongation; regulation of DNA recombination; RNA splicing; gene expression; intronless viral mRNA export from host nucleus; mRNA 3'-end processing; termination of RNA polymerase II transcription; replication fork processing