Rabbit CRYM Polyclonal Antibody | anti-CRYM antibody

CRYM Polyclonal Antibody

Cat. Num. AAA3008544

• Gene Names: CRYM; THBP; DFNA40
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CRYM; Polyclonal Antibody; CRYM Polyclonal Antibody; THBP; DFNA40; anti-CRYM antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CRYM polyclonal antibody detects endogenous levels of CRYM protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 314

• Applicable Applications for anti-CRYM antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human CRYM.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using CRYM antibody.)

Related Product Information for anti-CRYM antibody

Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness.

NCBI and Uniprot Product Information

• NCBI GI #: 2498259
• NCBI GeneID: 1428
• UniProt Accession #: Q14894
• Molecular Weight: ~ 34kDa
• NCBI Official Full Name: Ketimine reductase mu-crystallin
• NCBI Official Synonym Full Names: crystallin mu
• NCBI Official Symbol: CRYM
• NCBI Official Synonym Symbols: THBP; DFNA40
• NCBI Protein Information: ketimine reductase mu-crystallin
• UniProt Protein Name: Ketimine reductase mu-crystallin
• Protein Family: Ketimine reductase
• UniProt Gene Name: CRYM
• UniProt Synonym Gene Names: THBP
• UniProt Entry Name: CRYM_HUMAN

NCBI Description

Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. [provided by RefSeq, Sep 2014]

Uniprot Description

CRYM: Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors. Belongs to the ornithine cyclodeaminase family.

Protein type: Lyase; EC 1.5.1.25

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: mitochondrion; cytoplasm; nucleus

Molecular Function: thiomorpholine-carboxylate dehydrogenase activity; protein homodimerization activity; NADP binding; transcription corepressor activity

Biological Process: sensory perception of sound; negative regulation of transcription from RNA polymerase II promoter; thyroid hormone metabolic process

Disease: Deafness, Autosomal Dominant 40