Western Blot (WB)
(Western blot analysis of CRYAB (arrow) using rabbit polyclonal CRYAB Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CRYAB gene.)
Rabbit CRYAB Polyclonal Antibody | anti-CRYAB antibody
CRYAB Antibody (Center)
Western Blot (WB)
(Western blot analysis of CRYAB (arrow) using rabbit polyclonal CRYAB Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CRYAB gene.)
Western Blot (WB)
(Western blot analysis of CRYAB (arrow) using rabbit polyclonal CRYAB Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CRYAB gene.)
Western Blot (WB)
(CRYAB Antibody (Center) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the CRYAB antibody detected the CRYAB protein (arrow).)
Western Blot (WB)
(CRYAB Antibody (Center) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the CRYAB antibody detected the CRYAB protein (arrow).)
Immunohistochemistry (IHC)
(CRYA Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CRYA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CRYA Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CRYA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
taxon-specific, or enzyme, and ubiquitous. The latter class
constitutes the major proteins of vertebrate eye lens and maintains
the transparency and refractive index of the lens. Since lens
central fiber cells lose their nuclei during development, these
crystallins are made and then retained throughout life, making them
extremely stable proteins. Mammalian lens crystallins are divided
into alpha, beta, and gamma families; beta and gamma crystallins
are also considered as a superfamily. Alpha and beta families are
further divided into acidic and basic groups. Seven protein regions
exist in crystallins: four homologous motifs, a connecting peptide,
and N- and C-terminal extensions. Alpha crystallins are composed of
two gene products: alpha-A and alpha-B, for acidic and basic,
respectively. Alpha crystallins can be induced by heat shock and
are members of the small heat shock protein (sHSP also known as the
HSP20) family. They act as molecular chaperones although they do
not renature proteins and release them in the fashion of a true
chaperone; instead they hold them in large soluble aggregates.
Post-translational modifications decrease the ability to chaperone.
These heterogeneous aggregates consist of 30-40 subunits; the
alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two
additional functions of alpha crystallins are an autokinase
activity and participation in the intracellular architecture.
Alpha-A and alpha-B gene products are differentially expressed;
alpha-A is preferentially restricted to the lens and alpha-B is
expressed widely in many tissues and organs. Elevated expression of
alpha-B crystallin occurs in many neurological diseases; a missense
mutation cosegregated in a family with a desmin-related myopathy.
Jehle, S., et al. Nat. Struct. Mol. Biol. 17(9):1037-1042(2010)
Kida, E., et al. J. Neuropathol. Exp. Neurol. 69(7):745-759(2010)
Deng, Y., et al. BMB Rep 43(6):432-437(2010)
Houck, S.A., et al. PLoS ONE 5 (7), E11795 (2010) :
NCBI and Uniprot Product Information
NCBI Description
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Uniprot Description
CRYAB: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP also known as the HSP20) family. Alpha-B is expressed in the lens as well as other tissues. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
Protein type: Chaperone; Heat shock protein
Chromosomal Location of Human Ortholog: 11q22.3-q23.1
Cellular Component: microtubule cytoskeleton; Golgi apparatus; cell surface; mitochondrion; cytoplasm; plasma membrane; actin filament bundle; nucleus; Z disc; cytosol
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; metal ion binding; microtubule binding; unfolded protein binding; structural constituent of eye lens
Biological Process: lens development in camera-type eye; muscle development; protein folding; microtubule polymerization or depolymerization; stress-activated MAPK cascade; negative regulation of caspase activity; response to estradiol stimulus; tubulin folding; muscle contraction; response to hydrogen peroxide; negative regulation of intracellular transport; response to hypoxia; negative regulation of cell growth; protein homooligomerization; aging; negative regulation of apoptosis
Disease: Cataract 16, Multiple Types; Myopathy, Myofibrillar, Fatal Infantile Hypertonic, Alpha-b Crystallin-related; Myopathy, Myofibrillar, 2; Cardiomyopathy, Dilated, 1ii
Research Articles on CRYAB
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Product Notes
The CRYAB cryab (Catalog #AAA9214516) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 84-112. The CRYAB Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Bovine, Monkey, Pig, Rabbit, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's CRYAB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the CRYAB cryab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CRYAB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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