Rabbit anti-Human, Mouse Cry2 Polyclonal Antibody | anti-Cry2 antibody

Cry2, CT (Cryptochrome-2, KIAA0658) (AP)

Cat. Num. AAA6470711

• Gene Names: CRY2; HCRY2; PHLL2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Cry2; Polyclonal Antibody; CT (Cryptochrome-2; KIAA0658) (AP); anti-Cry2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CRY2. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-Cry2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CRY2 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hCry2-R579 in mouse liver tissue and HepG2 cell line lysates (35ug/lane). CRY2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-Cry2 antibody

Antibodies; Receptors

References

1. Ozgur, S., et al., Biochemistry 42(10):2926-2932 (2003). 2. Griffin, E.A. Jr., et al., Science 286(5440):768-771 (1999). 3. van der Horst, G.T., et al., Nature 398(6728):627-630 (1999). 4. Kobayashi, K., et al., Nucleic Acids Res. 26(22):5086-5092 (1998). 5. Hsu, D.S., et al., Biochemistry 35(44):13871-13877 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 118572252
• NCBI GeneID: 1408
• NCBI Accession #: NP_066940
• NCBI GenBank Nucleotide #: NM_021117
• UniProt Accession #: Q49AN0
• Molecular Weight: 67kDa
• NCBI Official Full Name: Cryptochrome-2
• NCBI Official Synonym Full Names: cryptochrome circadian regulator 2
• NCBI Official Symbol: CRY2
• NCBI Official Synonym Symbols: HCRY2; PHLL2
• NCBI Protein Information: cryptochrome-2
• UniProt Protein Name: Cryptochrome-2
• Protein Family: Cryptochrome
• UniProt Gene Name: CRY2
• UniProt Synonym Gene Names: KIAA0658
• UniProt Entry Name: CRY2_HUMAN

NCBI Description

This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]

Uniprot Description

CRY2: Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL. Belongs to the DNA photolyase class-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Lyase

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cytoplasm; extracellular region; nucleus

Molecular Function: ubiquitin binding; protein binding; DNA binding; DNA (6-4) photolyase activity; damaged DNA binding; deoxyribodipyrimidine photo-lyase activity; blue light photoreceptor activity; single-stranded DNA binding; phosphatase binding

Biological Process: circadian rhythm; photoreactive repair; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; regulation of circadian rhythm; protein-chromophore linkage; entrainment of circadian clock by photoperiod; blue light signaling pathway; negative regulation of phosphoprotein phosphatase activity; negative regulation of circadian rhythm; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent