Rabbit anti-Human CRTC2 Polyclonal Antibody | anti-CRTC2 antibody

CRTC2, NT (CRTC2, TORC2, CREB-regulated transcription coactivator 2, Transducer of regulated cAMP response element-binding protein 2) (APC)

Cat. Num. AAA6288979

• Gene Names: CRTC2; TORC2; TORC-2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CRTC2; Polyclonal Antibody; NT (CRTC2; TORC2; CREB-regulated transcription coactivator 2; Transducer of regulated cAMP response element-binding protein 2) (APC); Transducer of regulated cAMP response element-binding protein 2; anti-CRTC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CRTC2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CRTC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-123 amino acids from the N-terminal region of human CRTC2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CRTC2 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CRTC2 antibody detected the CRTC2 protein (arrow).)

Immunohistochemistry (IHC)

(CRTC2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CRTC2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CRTC2 antibody

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).

Product Categories/Family for anti-CRTC2 antibody

Antibodies; Binding Proteins

References

Nakatsu, Y., et al. J. Biol. Chem. 285(43):33018-33027(2010). Kaizuka, T., et al. J. Biol. Chem. 285(26):20109-20116(2010). Lyo, D., et al. Biochem. Biophys. Res. Commun. 396(2):562-565(2010). Lu, M., et al. J. Am. Soc. Nephrol. 21(5):811-818(2010). Roulin, D., et al. Mol. Cancer 9, 57 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 32171215
• NCBI GeneID: 200186
• NCBI Accession #: NP_859066.1
• NCBI GenBank Nucleotide #: NM_181715.2
• UniProt Accession #: Q53ET0; Q6UUV8; Q7Z3X7; Q8N332
• Molecular Weight: 73,302 Da
• NCBI Official Full Name: CREB-regulated transcription coactivator 2
• NCBI Official Synonym Full Names: CREB regulated transcription coactivator 2
• NCBI Official Symbol: CRTC2
• NCBI Official Synonym Symbols: TORC2; TORC-2
• NCBI Protein Information: CREB-regulated transcription coactivator 2; transducer of regulated cAMP response element-binding protein (CREB) 2
• UniProt Protein Name: CREB-regulated transcription coactivator 2
• Protein Family: CREB-regulated transcription coactivator
• UniProt Gene Name: CRTC2
• UniProt Synonym Gene Names: TORC2; TORC-2; Transducer of CREB protein 2
• UniProt Entry Name: CRTC2_HUMAN

NCBI Description

This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2010]

Uniprot Description

TORC2: the transducer of regulated CREB protein 2 (TORC2) is a cAMP responsive coactivator that, in concert with LKB1 and AMPK, controls glucose homeostasis in the liver. Under fasting conditions, cytoplasmic TORC2 is transported to the nucleus where it binds to CREB and stimulates gluconeogenisis. Re-feeding and the ensuing insulin response inhibits gluconeogenic gene expression by promoting the phosphorylation, cytoplasmic export and degradation of TORC2. TORC2 is phosphorylated by the kinase QIK which in turn is activated by phosphorylation at Ser358 by insulin-activated Akt2. Phosphorylated TORC2 is subsequently ubiquitinylated at Lys 628 and degraded by the 26S proteasome. TORC2 protein levels and activity are increased in diabetes owing to a block in TORC2 phosphorylation, implicating this pathway in the pathogenesis of diabetes. The CREB/TORC2 regulatory axis controls the normal pattern of germinal center (GC) B cell gene activation/repression that promotes B cell development and circumvents GC lymphomagenesis.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; cAMP response element binding protein binding

Biological Process: viral reproduction; transcription, DNA-dependent; activation of CREB transcription factor; positive regulation of transcription from RNA polymerase II promoter; glucose homeostasis; gluconeogenesis; protein homotetramerization