Rabbit anti-Human, Mouse CROT Polyclonal Antibody | anti-CROT antibody

CROT, NT (CROT, COT, Peroxisomal carnitine O-octanoyltransferase) (HRP)

Cat. Num. AAA6288938

• Gene Names: CROT; COT
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CROT; Polyclonal Antibody; NT (CROT; COT; Peroxisomal carnitine O-octanoyltransferase) (HRP); Peroxisomal carnitine O-octanoyltransferase; anti-CROT antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CROT. Species Crossreactivity: mouse
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CROT antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: CROT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57~86 amino acids from the N-terminal region of human CROT.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Flow Cytometry (FC/FACS)

(CROT Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with CROT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of CROT Antibody (N-term) in K562 cell line and mouse liver tissue lysates (35ug/lane). CROT (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-CROT antibody

Antibodies; Enzymes

References

Li, Y.L., et al. FEBS J. 276(1):303-314(2009). Morillas, M., et al. J. Biol. Chem. 277(13):11473-11480(2002). van der Leij, F.R., et al. Mol. Genet. Metab. 71 (1-2), 139-153 (2000): Ferdinandusse, S., et al. Biochem. Biophys. Res. Commun. 263(1):213-218(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 48429265
• NCBI GeneID: 54677
• UniProt Accession #: Q9UKG9; Q86V17; Q8IUW9; Q9Y6I2; A4D1D6; E7EQF2
• Molecular Weight: 612
• NCBI Official Full Name: Peroxisomal carnitine O-octanoyltransferase
• NCBI Official Synonym Full Names: carnitine O-octanoyltransferase
• NCBI Official Symbol: CROT
• NCBI Official Synonym Symbols: COT
• NCBI Protein Information: peroxisomal carnitine O-octanoyltransferase; peroxisomal carnitine acyltransferase
• UniProt Protein Name: Peroxisomal carnitine O-octanoyltransferase
• Protein Family: Crotasin
• UniProt Gene Name: CROT
• UniProt Synonym Gene Names: COT; COT
• UniProt Entry Name: OCTC_HUMAN

NCBI Description

This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.[provided by RefSeq, Jan 2009]

Uniprot Description

CROT: Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl- CoA, to its corresponding carnitine ester. Belongs to the carnitine/choline acetyltransferase family.

Protein type: EC 2.3.1.137; Transferase

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: peroxisomal matrix; intracellular membrane-bound organelle; mitochondrion; peroxisome

Molecular Function: carnitine O-octanoyltransferase activity; receptor binding

Biological Process: coenzyme A metabolic process; fatty acid beta-oxidation using acyl-CoA oxidase; generation of precursor metabolites and energy; fatty acid beta-oxidation; carnitine metabolic process; cellular lipid metabolic process; fatty acid metabolic process; medium-chain fatty acid metabolic process; fatty acid transport