Rabbit CROT Polyclonal Antibody | anti-CROT antibody

CROT Rabbit pAb

Cat. Num. AAA4758911

• Gene Names: CROT; COT
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: CROT; Polyclonal Antibody; CROT Rabbit pAb; carnitine O-octanoyltransferase; COT; anti-CROT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: 0.8mg/ml (varies by lot)

• Applicable Applications for anti-CROT antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:1000-1:5000; IHC-P: 1:150-1:300
• Immunogen: Fusion protein of human CROT
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of CROT in Mouse liver tissue, Mouse kidney tissue lysates in order using CROT Rabbit pAb at dilution 1/800)

Immunohistochemistry (IHC)

(Immunohistochemistry of CROT in paraffin-embedded Human esophagus cancer tissue using CROT Rabbit pAb at dilution 1/115. Predicted cell location: Cytoplasm)

Related Product Information for anti-CROT antibody

This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4, 8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.

NCBI and Uniprot Product Information

• NCBI GI #: 48429265
• NCBI GeneID: 54677
• UniProt Accession #: Q9UKG9; Q86V17; Q8IUW9; Q9Y6I2; A4D1D6; E7EQF2
• Molecular Weight: 612
• NCBI Official Full Name: Peroxisomal carnitine O-octanoyltransferase
• NCBI Official Synonym Full Names: carnitine O-octanoyltransferase
• NCBI Official Symbol: CROT
• NCBI Official Synonym Symbols: COT
• NCBI Protein Information: peroxisomal carnitine O-octanoyltransferase; peroxisomal carnitine acyltransferase
• UniProt Protein Name: Peroxisomal carnitine O-octanoyltransferase
• Protein Family: Crotasin
• UniProt Gene Name: CROT
• UniProt Synonym Gene Names: COT; COT
• UniProt Entry Name: OCTC_HUMAN

NCBI Description

This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.[provided by RefSeq, Jan 2009]

Uniprot Description

CROT: Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl- CoA, to its corresponding carnitine ester. Belongs to the carnitine/choline acetyltransferase family.

Protein type: EC 2.3.1.137; Transferase

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: peroxisomal matrix; intracellular membrane-bound organelle; mitochondrion; peroxisome

Molecular Function: carnitine O-octanoyltransferase activity; receptor binding

Biological Process: coenzyme A metabolic process; fatty acid beta-oxidation using acyl-CoA oxidase; generation of precursor metabolites and energy; fatty acid beta-oxidation; carnitine metabolic process; cellular lipid metabolic process; fatty acid metabolic process; medium-chain fatty acid metabolic process; fatty acid transport