Rabbit Crk Polyclonal Antibody | anti-Crk antibody

Crk (Tyr-251), phospho-specific Antibody

Cat. Num. AAA474286

• Reactivity: Human, Rat, Mouse, Chicken, Fish
• Applications: Western Blot, ELISA, Immunohistochemistry
• Synonyms: Crk; Polyclonal Antibody; Crk (Tyr-251); phospho-specific Antibody; phospho-specific; Crk Tyr251; anti-Crk antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Chicken, Fish
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using phospho-Crk (Tyr-251) peptide (without carrier). The antibody detects a 38 and 40kDa* proteins corresponding to the molecular mass of Crk II and CrkL on SDS-PAGE immunoblots of human K562 cells stimulated with pervanadate and A431 cells stimulated with EGF. This reactivity is not observed after akaline phosphatase treatment.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-Crk antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:2000; WB: 1:1000; IHC: 1:100
• Immunogen: Phospho-Crk (Tyr-251) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-251 in human Crk II. This peptide sequence is well conserved in mouse and rat Crk II, as well as in CrkL (Tyr-251). The site is not found in Crk I.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of human K562 cells stimulated with pervanadate (1mM) for 30 min. (lanes 1-6). The blot was treated with akaline phosphatase to dephosphorylate Crk (lanes 2, 4 & 6), then the blot was probed with rabbit polyclonals CrkL (C-terminus) CP3081 (lanes 1 & 2), Crk (Tyr-251) phospho-specific CP3091 (lanes 3 & 4), and mouse monoclonal anti-Crk II (C-terminal region) (lanes 5 & 6).)

Related Product Information for anti-Crk antibody

The Crk family of adaptor proteins (Crk I, Crk II and CrkL) are Src Homology 2 (SH2) and Src Homology 3 (SH3) domain-containing proteins that form protein complexes important for transmiting signals downstream of tyrosine kinases. Both Crk II and CrkL are composed of a single SH2 domain, followed by two tandem SH3 domains. Crk II is also alternatively spliced to a minor product, Crk I, which is structurally and functionally more similar to the v-Crk oncogene. Both Crk II and CrkL are ubiquitously expressed and their SH domains are highly homologous, however both are required for mouse development and have distinct non-overlapping phenotypes in knockout mice. Phosphorylation may be important for regulating Crk activity. Crk II Tyr-221 (CrkL Tyr-207) phosphorylation is a negative regulatory site, while Crk Tyr-251 phosphorylation in the SH3 domain is a positive regulatory site. EGF stimulation induces phosphorylation of Tyr-251, which increases binding of Crk to the SH2 domain of Abl, and promotes transactivation of Abl.

References

Sriram, G. et al. (2011) Oncogene 30(46):4645.Feller, S.M. & Lewitzky, M. (2006) Curr.
Pharm. Des. 12(5):529.
Feller, S.M. (2001) Oncogene. 20(44):6348.

NCBI and Uniprot Product Information

• NCBI GI #: 4885153
• NCBI GeneID: 1399
• NCBI Accession #: NP_005198.1
• NCBI GenBank Nucleotide #: NM_005207.3
• UniProt Accession #: P46109; A8KA44; D3DX35
• Molecular Weight: 33,777 Da
• NCBI Official Full Name: crk-like protein
• NCBI Official Synonym Full Names: v-crk sarcoma virus CT10 oncogene homolog (avian)-like
• NCBI Official Symbol: CRKL
• NCBI Protein Information: crk-like protein
• UniProt Protein Name: Crk-like protein
• Protein Family: Crk-like protein
• UniProt Gene Name: CRKL
• UniProt Entry Name: CRKL_HUMAN

NCBI Description

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]

Uniprot Description

CRKL: an oncogenic adaptor protein with an SH2-SH3-SH3 domain structure. Plays a specific role in integrin-induced migration as a downstream mediator of Src. Activates the RAS and Jnk signaling pathways. Transforms fibroblasts in a RAS-dependent fashion. A substrate of BCR-ABL and plays a role in fibroblast transformation by BCR-ABL.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytosol; endosome

Molecular Function: protein binding; signal transducer activity; SH3/SH2 adaptor activity

Biological Process: anterior/posterior pattern formation; blood vessel development; organ morphogenesis; nerve growth factor receptor signaling pathway; activation of MAPKK activity; thymus development; heart development; Ras protein signal transduction; JNK cascade; parathyroid gland development