Western Blot (WB)
(The anti-CBX4 C-term Pab is used in Western blot to detect CBX4 in Jurkat cell lysate.)
Rabbit CBX4 Polyclonal Antibody | anti-CBX4 antibody
CBX4 Antibody (C-term)
Gene Names
CBX4; PC2; NBP16
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CBX4; Polyclonal Antibody; CBX4 Antibody (C-term); E3 SUMO-protein ligase CBX4; 632-; Chromobox protein homolog 4; Polycomb 2 homolog; Pc2; hPc2; anti-CBX4 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CBX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-437 amino acids from the C-terminal region of human CBX4.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
410-437
Sequence Length
290
Applicable Applications for anti-CBX4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(The anti-CBX4 C-term Pab is used in Western blot to detect CBX4 in Jurkat cell lysate.)
Western Blot (WB)
(The anti-CBX4 C-term Pab is used in Western blot to detect CBX4 in Jurkat cell lysate.)
Related Product Information for anti-CBX4 antibody
Chromobox homolog 4 (CBX4 or Pc2) is a member of Drosophila Polycomb group gene family. The polycomb group (PcG) genes are essential for maintenance of appropriate expression patterns of developmental master regulators and thus are essential for proper development. Changes in expression pf PcG proteins have been associated with cancer. CBX4 is a part of the cellular memory system that is responsible for the inheritance of gene activity by progeny cells. CBX4 is involved in maintaining the transcriptionally repressive state of genes. It modifies chromatin, rendering it heritably changed in its expressibility. Structurally, CBX4 contains 1 chromo domain. It was reported that Pc2 is involved in sumoylation process by recruiting SUMO E1 and E2, and Pc2 is actually SUMO E3 during sumoylation events. CBX4 expression is ubiquitous.
References
Kagey, M.H., et al., Cell 113(1):127-137 (2003).
Satijn, D.P., et al., Mol. Cell. Biol. 17(10):6076-6086 (1997).
Satijn, D.P., et al., Mol. Cell. Biol. 17(10):6076-6086 (1997).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61368
NCBI Official Full Name
E3 SUMO-protein ligase CBX4
NCBI Official Synonym Full Names
chromobox homolog 4
NCBI Official Symbol
CBX4
NCBI Official Synonym Symbols
PC2; NBP16
NCBI Protein Information
E3 SUMO-protein ligase CBX4
UniProt Protein Name
E3 SUMO-protein ligase CBX4
Protein Family
UniProt Gene Name
CBX4
UniProt Synonym Gene Names
Pc2; hPc2
UniProt Entry Name
CBX4_HUMAN
Uniprot Description
CBX4: E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.-; Transcription, coactivator/corepressor; SUMO ligase
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: nucleoplasm; nuclear speck; PcG protein complex; nucleus
Molecular Function: protein binding; single-stranded RNA binding; enzyme binding; SUMO binding; phosphoprotein binding; chromatin binding; transcription corepressor activity; methylated histone residue binding; ligase activity
Biological Process: protein sumoylation; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
Research Articles on CBX4
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Product Notes
The CBX4 cbx4 (Catalog #AAA9212104) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 410-437. The CBX4 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's CBX4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the CBX4 cbx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CBX4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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